Detailed information of TA system TA024668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1275402..1276015
• Replicon: chromosome
• Accession: NC_013960
• Organism: Nitrosococcus halophilus Nc 4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: D5C0B4
• Locus tag: NHAL_RS06305
• Protein ID: WP_013032331.1
• Coordinates: 1275614..1276015 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D5C0B3
• Locus tag: NHAL_RS06300
• Protein ID: WP_013032330.1
• Coordinates: 1275402..1275617 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NHAL_RS06270	1270954..1271529	-	576	WP_157862491.1	hypothetical protein	-
NHAL_RS06275	1271718..1271945	-	228	WP_013032326.1	hypothetical protein	-
NHAL_RS06280	1271949..1272215	-	267	WP_013032327.1	zinc ribbon domain-containing protein	-
NHAL_RS06285	1272427..1273392	+	966	WP_013032328.1	integron integrase	-
NHAL_RS06290	1273443..1274594	-	1152	WP_013032329.1	IS4 family transposase	-
NHAL_RS06295	1274799..1275005	-	207	Protein_1241	type II toxin-antitoxin system HicB family antitoxin	-
NHAL_RS06300	1275402..1275617	+	216	WP_013032330.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
NHAL_RS06305	1275614..1276015	+	402	WP_013032331.1	PIN domain nuclease	Toxin
NHAL_RS06310	1276071..1278209	-	2139	WP_013032332.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-122)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (7-52)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15189.60 Da
• Isoelectric Point: 6.7504

>T24668 WP_013032331.1 NC_013960:1275614-1276015 [Nitrosococcus halophilus Nc 4]
MTLVDTSVWIDFFRGHDLPHVQRLEEIIGQGEDLALCGVILTEILQGIRDDQTFSRTQTRLEPLLLLPMTQSVFIQAAII
YRGLRAKGMTIRKPVDCMIAATALEHGAWLLHNDRDFEAIARHYPLRSSNLRH

Nucleotide

• Length: 402 bp

>T24668 NC_013960:1275614-1276015 [Nitrosococcus halophilus Nc 4]
ATGACCTTAGTGGATACCAGTGTTTGGATCGACTTTTTCCGTGGCCATGACCTGCCGCACGTACAACGGCTGGAGGAGAT
AATAGGGCAGGGCGAGGACTTGGCCCTATGCGGAGTGATACTGACTGAGATCCTCCAGGGGATTCGCGACGATCAGACCT
TCAGCCGCACTCAAACCCGACTGGAGCCCCTGCTGTTGTTGCCCATGACCCAATCAGTCTTTATCCAAGCAGCGATTATT
TATCGCGGCCTCCGCGCTAAAGGCATGACTATTCGTAAGCCAGTGGACTGCATGATCGCCGCTACCGCTTTGGAGCATGG
GGCATGGCTGCTCCATAATGACCGTGATTTCGAGGCCATCGCCCGGCATTATCCCCTGCGGAGCAGTAATCTCAGGCATT
GA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8189.42 Da
• Isoelectric Point: 5.8573

>AT24668 WP_013032330.1 NC_013960:1275402-1275617 [Nitrosococcus halophilus Nc 4]
MSHSGQRTNIVIDPELIEQGLRVTGLKTRRELVDFALRELLRRENQKKLLELKGQVEWEGDLGAMRGEVCE

Nucleotide

• Length: 216 bp

>AT24668 NC_013960:1275402-1275617 [Nitrosococcus halophilus Nc 4]
ATGAGTCATTCAGGACAGCGTACCAATATCGTCATCGATCCTGAGCTGATCGAACAGGGCCTGCGTGTCACCGGCCTTAA
GACCCGCCGCGAGCTTGTCGACTTCGCCCTGCGCGAACTGTTACGCCGCGAGAATCAAAAGAAGCTGTTGGAACTCAAAG
GACAGGTGGAGTGGGAGGGGGATCTAGGCGCGATGCGCGGAGAGGTCTGTGAATGA

Structures

Toxin

• AlphaFold DB: D5C0B4

Antitoxin

• AlphaFold DB: D5C0B3