Detailed information of TA system TA024666

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicA
• Location: 1013666..1014077
• Replicon: chromosome
• Accession: NC_013960
• Organism: Nitrosococcus halophilus Nc 4

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D5BYY8
• Locus tag: NHAL_RS05040
• Protein ID: WP_013032093.1
• Coordinates: 1013892..1014077 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: D5BYY7
• Locus tag: NHAL_RS05035
• Protein ID: WP_013032092.1
• Coordinates: 1013666..1013881 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NHAL_RS05010	1008760..1009473	-	714	WP_013032086.1	OmpA family protein	-
NHAL_RS05015	1009482..1011302	-	1821	WP_013032087.1	hypothetical protein	-
NHAL_RS05020	1011281..1011667	-	387	WP_013032088.1	hypothetical protein	-
NHAL_RS05025	1011835..1012608	-	774	WP_013032089.1	DCL family protein	-
NHAL_RS20160	1012715..1012942	-	228	WP_041355426.1	carbon storage regulator	-
NHAL_RS05035	1013666..1013881	-	216	WP_013032092.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
NHAL_RS05040	1013892..1014077	-	186	WP_013032093.1	type II toxin-antitoxin system HicA family toxin	Toxin
NHAL_RS05045	1014284..1015216	-	933	WP_013032094.1	hypothetical protein	-
NHAL_RS05050	1015316..1016038	-	723	WP_013032095.1	DUF2971 domain-containing protein	-
NHAL_RS20165	1017255..1017434	+	180	WP_083761359.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	950230..1029307	79077

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-61)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6702.86 Da
• Isoelectric Point: 9.6903

>T24666 WP_013032093.1 NC_013960:c1014077-1013892 [Nitrosococcus halophilus Nc 4]
MKVEEVIQMLEYEGGYLVRVRGSHCQFKHPEKPGKVTVAGKPSVDVPPGTLNNILKQAGLK

Nucleotide

• Length: 186 bp

>T24666 NC_013960:c1014077-1013892 [Nitrosococcus halophilus Nc 4]
ATGAAAGTAGAGGAGGTCATTCAAATGCTTGAGTACGAGGGTGGGTATCTCGTTCGGGTACGAGGTAGCCACTGCCAGTT
CAAGCATCCCGAAAAGCCCGGAAAAGTCACCGTGGCGGGCAAACCCAGCGTGGATGTTCCGCCGGGGACGCTCAATAATA
TTCTGAAGCAGGCGGGTCTAAAGTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7857.89 Da
• Isoelectric Point: 4.1438

>AT24666 WP_013032092.1 NC_013960:c1013881-1013666 [Nitrosococcus halophilus Nc 4]
MRYRVVIERGPKSWGAYVPDLPGCAAVGETKEEVLELIQGAIELHLEELRADGEPLPEPHSEVDYVEVSAI

Nucleotide

• Length: 216 bp

>AT24666 NC_013960:c1013881-1013666 [Nitrosococcus halophilus Nc 4]
ATGAGATATAGGGTCGTGATTGAGCGAGGCCCCAAGAGCTGGGGCGCGTATGTGCCCGATCTTCCCGGCTGTGCCGCTGT
AGGGGAGACGAAAGAGGAGGTTTTAGAGCTTATCCAGGGGGCAATTGAGCTTCACTTGGAAGAGCTTAGAGCGGACGGCG
AACCCCTCCCCGAGCCCCATTCGGAGGTGGACTATGTGGAGGTATCCGCCATCTAG

Structures

Toxin

• AlphaFold DB: D5BYY8

Antitoxin

• AlphaFold DB: D5BYY7