Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2066614..2067239 Replicon chromosome
Accession NZ_CP098131
Organism Klebsiella pneumoniae strain Z0117KP0028

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NBY54_RS09860 Protein ID WP_002882817.1
Coordinates 2066614..2066997 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NBY54_RS09865 Protein ID WP_004150355.1
Coordinates 2066997..2067239 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NBY54_RS09845 (2063980) 2063980..2064882 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NBY54_RS09850 (2064879) 2064879..2065514 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NBY54_RS09855 (2065511) 2065511..2066440 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NBY54_RS09860 (2066614) 2066614..2066997 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NBY54_RS09865 (2066997) 2066997..2067239 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NBY54_RS09870 (2067444) 2067444..2068361 + 918 WP_021466676.1 alpha/beta hydrolase -
NBY54_RS09875 (2068375) 2068375..2069316 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NBY54_RS09880 (2069361) 2069361..2069798 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NBY54_RS09885 (2069795) 2069795..2070655 - 861 WP_004146232.1 virulence factor BrkB family protein -
NBY54_RS09890 (2070649) 2070649..2071248 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T246642 WP_002882817.1 NZ_CP098131:c2066997-2066614 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT246642 WP_004150355.1 NZ_CP098131:c2067239-2066997 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References