Detailed information of TA system TA024664

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 632473..633063
• Replicon: chromosome
• Accession: NC_013960
• Organism: Nitrosococcus halophilus Nc 4

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D5BWS6
• Locus tag: NHAL_RS03060
• Protein ID: WP_013031702.1
• Coordinates: 632875..633063 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: D5BWS5
• Locus tag: NHAL_RS03055
• Protein ID: WP_013031701.1
• Coordinates: 632473..632871 (-)
• Length: 133 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NHAL_RS03030	627982..628911	-	930	WP_013031696.1	sulfotransferase	-
NHAL_RS03035	628902..630206	-	1305	WP_013031697.1	flippase	-
NHAL_RS03040	631007..631276	-	270	WP_013031698.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
NHAL_RS03045	631585..631812	+	228	WP_013031699.1	ribbon-helix-helix domain-containing protein	-
NHAL_RS03050	631813..632022	+	210	WP_013031700.1	hypothetical protein	-
NHAL_RS03055	632473..632871	-	399	WP_013031701.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
NHAL_RS03060	632875..633063	-	189	WP_013031702.1	type II toxin-antitoxin system HicA family toxin	Toxin
NHAL_RS03065	633191..633616	-	426	WP_013031703.1	nucleotidyltransferase substrate binding protein	-
NHAL_RS03070	633604..633955	-	352	Protein_609	nucleotidyltransferase domain-containing protein	-
NHAL_RS03075	634089..634703	+	615	WP_013031705.1	IS607 family transposase	-
NHAL_RS03080	634681..635835	+	1155	WP_013031706.1	transposase	-
NHAL_RS03085	636013..636324	+	312	WP_013031708.1	nucleotidyltransferase domain-containing protein	-
NHAL_RS03090	636321..636596	+	276	WP_203434354.1	hypothetical protein	-
NHAL_RS03095	636557..637780	-	1224	WP_013031487.1	IS256 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	615715..638766	23051

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-123)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7420.65 Da
• Isoelectric Point: 11.6577

>T24664 WP_013031702.1 NC_013960:c633063-632875 [Nitrosococcus halophilus Nc 4]
MNSTELIQRLEREGWKRVGGKGDHMKFKHPVKHNHVVVPHPRKDIPIGTLRNIYRQAGWKWR

Nucleotide

• Length: 189 bp

>T24664 NC_013960:c633063-632875 [Nitrosococcus halophilus Nc 4]
ATGAATAGCACGGAACTGATACAGCGGCTGGAGCGCGAAGGGTGGAAGCGAGTAGGTGGCAAGGGAGATCATATGAAGTT
CAAACATCCTGTGAAACACAATCATGTGGTCGTACCACATCCGCGCAAGGATATCCCTATCGGCACGCTACGCAATATCT
ACCGGCAAGCTGGATGGAAGTGGAGGTAA

Antitoxin

Protein

• Length: 133 a.a.
• Molecular weight: 15082.28 Da
• Isoelectric Point: 5.2493

>AT24664 WP_013031701.1 NC_013960:c632871-632473 [Nitrosococcus halophilus Nc 4]
MLYPVYIHPGDNKHAHGVTFPDFPGCFSAADSWEELPAKIQEAVELYFEGETMEIPPPTPLEELVQRPEFEGGVWMMVDI
DFSRVRPKAVRINISLPEALVRRIDEYAKAHQMSRSGFLAKVAEEAMRKKHN

Nucleotide

• Length: 399 bp

>AT24664 NC_013960:c632871-632473 [Nitrosococcus halophilus Nc 4]
ATGTTGTATCCGGTCTATATTCATCCAGGTGATAATAAGCATGCCCACGGAGTAACCTTTCCGGATTTCCCCGGATGTTT
CTCTGCGGCTGATTCCTGGGAGGAACTGCCCGCCAAAATTCAGGAGGCAGTAGAGCTGTATTTCGAGGGAGAGACAATGG
AGATCCCACCTCCCACACCGCTGGAGGAATTGGTGCAGCGCCCAGAATTCGAGGGCGGTGTGTGGATGATGGTGGATATT
GATTTCTCCCGCGTACGCCCCAAGGCGGTGCGCATCAATATCTCTCTGCCCGAAGCCTTGGTGCGCCGCATCGATGAATA
TGCAAAGGCACACCAGATGAGCCGTTCCGGATTTCTGGCAAAGGTCGCCGAAGAGGCAATGCGTAAGAAGCACAACTAA

Structures

Toxin

• AlphaFold DB: D5BWS6

Antitoxin

• AlphaFold DB: D5BWS5