Detailed information of TA system TA024662

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 275316..275898
• Replicon: chromosome
• Accession: NC_013960
• Organism: Nitrosococcus halophilus Nc 4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5BUR8
• Locus tag: NHAL_RS01340
• Protein ID: WP_013031364.1
• Coordinates: 275316..275651 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: D5BUR9
• Locus tag: NHAL_RS01345
• Protein ID: WP_013031365.1
• Coordinates: 275656..275898 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NHAL_RS01320	270754..271335	-	582	WP_013031359.1	hypothetical protein	-
NHAL_RS01325	271872..272210	+	339	WP_157862457.1	hypothetical protein	-
NHAL_RS01330	272234..273952	-	1719	WP_013031362.1	chloride channel protein	-
NHAL_RS01335	274294..274509	-	216	WP_013031363.1	type II toxin-antitoxin system HicB family antitoxin	-
NHAL_RS01340	275316..275651	-	336	WP_013031364.1	endoribonuclease MazF	Toxin
NHAL_RS01345	275656..275898	-	243	WP_013031365.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
NHAL_RS01350	276138..277076	-	939	WP_013031366.1	50S ribosomal protein L3 N(5)-glutamine methyltransferase	-
NHAL_RS01355	277088..278497	-	1410	WP_013031367.1	3-isopropylmalate dehydratase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

• SpoVT-AbrB domain (6-50)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12381.46 Da
• Isoelectric Point: 9.5483

>T24662 WP_013031364.1 NC_013960:c275651-275316 [Nitrosococcus halophilus Nc 4]
MAYVPNRGDVVWLDFNPQAGHEQAGRRPALALSPLAYNRKVGLAIFCPITNQTKGYPFEVVIPDGYQVTGVILSDQIKNL
DWKNRSCRRVCVLEAAIVKDVLKKLSVLLQT

Nucleotide

• Length: 336 bp

>T24662 NC_013960:c275651-275316 [Nitrosococcus halophilus Nc 4]
ATGGCTTATGTTCCTAATCGGGGTGACGTCGTATGGTTGGATTTCAACCCACAGGCGGGGCACGAGCAAGCCGGGAGAAG
GCCAGCGTTAGCGCTCTCTCCCCTAGCGTATAACAGGAAAGTGGGGTTGGCCATTTTTTGTCCGATCACTAACCAAACCA
AAGGCTATCCTTTCGAGGTCGTGATTCCTGATGGTTACCAGGTCACCGGAGTCATTCTTTCGGACCAAATCAAGAACTTG
GATTGGAAGAACAGGAGTTGCAGGCGCGTCTGCGTGCTAGAAGCTGCGATAGTTAAAGATGTGCTAAAGAAATTATCGGT
ATTGTTGCAGACATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9053.41 Da
• Isoelectric Point: 5.6663

>AT24662 WP_013031365.1 NC_013960:c275898-275656 [Nitrosococcus halophilus Nc 4]
MKIQIQKWGNSLAVRIPKSFAQEVHMDQGTIVDLALEKGQIIIRPVVEPQYSLNELLSKVTKENIHSEADTGEPQGKEIW

Nucleotide

• Length: 243 bp

>AT24662 NC_013960:c275898-275656 [Nitrosococcus halophilus Nc 4]
ATGAAGATACAGATTCAGAAGTGGGGAAATAGCTTAGCAGTACGTATCCCCAAATCATTTGCTCAAGAAGTCCATATGGA
TCAGGGCACAATTGTTGATTTGGCGCTAGAAAAGGGTCAAATCATCATCAGGCCTGTGGTTGAGCCACAGTATTCGCTAA
ATGAATTATTATCCAAAGTGACCAAGGAAAACATTCATAGCGAAGCCGATACCGGGGAACCTCAAGGAAAAGAGATTTGG
TGA

Structures

Toxin

• AlphaFold DB: D5BUR8

Antitoxin

• AlphaFold DB: D5BUR9