Detailed information of TA system TA024659

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicA
• Location: 130366..130773
• Replicon: chromosome
• Accession: NC_013960
• Organism: Nitrosococcus halophilus Nc 4

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D5C4R4
• Locus tag: NHAL_RS00600
• Protein ID: WP_013031233.1
• Coordinates: 130588..130773 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: D5C4R3
• Locus tag: NHAL_RS00595
• Protein ID: WP_013031232.1
• Coordinates: 130366..130575 (-)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NHAL_RS00575	125718..126215	-	498	WP_013031228.1	hypothetical protein	-
NHAL_RS00580	126606..127514	-	909	WP_013031229.1	complex I NDUFA9 subunit family protein	-
NHAL_RS19555	127669..129147	-	1479	WP_013031230.1	PAS domain-containing sensor histidine kinase	-
NHAL_RS00590	129549..129761	-	213	WP_013031231.1	cold-shock protein	-
NHAL_RS20855	129994..130143	-	150	WP_157862447.1	hypothetical protein	-
NHAL_RS00595	130366..130575	-	210	WP_013031232.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
NHAL_RS00600	130588..130773	-	186	WP_013031233.1	type II toxin-antitoxin system HicA family toxin	Toxin
NHAL_RS21420	130936..131304	-	369	WP_203434342.1	hypothetical protein	-
NHAL_RS00610	131623..131886	-	264	WP_013031234.1	Txe/YoeB family addiction module toxin	-
NHAL_RS00615	131883..132125	-	243	WP_013031235.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
NHAL_RS00620	132229..132534	-	306	WP_013031236.1	hypothetical protein	-
NHAL_RS00625	132531..132809	-	279	WP_013031237.1	toxin	-
NHAL_RS00630	133046..133438	-	393	Protein_123	transposase	-
NHAL_RS00635	133537..133797	-	261	WP_013031239.1	DUF2442 domain-containing protein	-
NHAL_RS20025	133757..133996	-	240	WP_013031240.1	DUF4160 domain-containing protein	-
NHAL_RS00640	134132..134398	-	267	WP_013031241.1	DUF2442 domain-containing protein	-
NHAL_RS00645	134406..134672	-	267	Protein_127	DUF4160 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-61)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6797.85 Da
• Isoelectric Point: 10.8214

>T24659 WP_013031233.1 NC_013960:c130773-130588 [Nitrosococcus halophilus Nc 4]
MKVGDILQKLKDDGWYLHSIRGSHRQFKHPSKLGRVTVPGKPGDDLAPGTVNSIFKQAGWK

Nucleotide

• Length: 186 bp

>T24659 NC_013960:c130773-130588 [Nitrosococcus halophilus Nc 4]
ATGAAAGTCGGTGATATTCTCCAAAAATTGAAAGACGACGGCTGGTACCTCCACTCAATTCGGGGTAGCCATCGCCAGTT
CAAGCACCCGTCCAAGTTGGGCCGGGTCACAGTTCCGGGAAAGCCTGGTGATGATCTGGCACCCGGAACCGTCAATAGCA
TTTTCAAGCAAGCTGGCTGGAAATAG

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 7589.49 Da
• Isoelectric Point: 3.8480

>AT24659 WP_013031232.1 NC_013960:c130575-130366 [Nitrosococcus halophilus Nc 4]
MKYAIVIEKAASNYSAYVPDLPGCVATGSTIEEVESEIREAIEFHLEGMREDGELIPPPSSQVEYVDIT

Nucleotide

• Length: 210 bp

>AT24659 NC_013960:c130575-130366 [Nitrosococcus halophilus Nc 4]
ATGAAATATGCAATCGTTATTGAAAAGGCTGCAAGCAACTATTCTGCCTATGTCCCAGACCTCCCTGGGTGCGTAGCTAC
CGGTTCTACCATTGAGGAAGTCGAATCAGAGATTCGGGAAGCCATTGAATTTCATCTTGAAGGAATGCGAGAGGATGGTG
AGCTAATTCCTCCACCAAGCAGTCAAGTTGAATACGTTGATATCACCTAA

Structures

Toxin

• AlphaFold DB: D5C4R4

Antitoxin

• AlphaFold DB: D5C4R3