Detailed information of TA system TA024658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 2817425..2818107
• Replicon: chromosome
• Accession: NC_013959
• Organism: Sideroxydans lithotrophicus ES-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D5CPM3
• Locus tag: SLIT_RS13945
• Protein ID: WP_013030914.1
• Coordinates: 2817425..2817709 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: D5CPM4
• Locus tag: SLIT_RS13950
• Protein ID: WP_013030915.1
• Coordinates: 2817706..2818107 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SLIT_RS13915	2813308..2813634	+	327	WP_013030462.1	transposase	-
SLIT_RS13920	2813631..2814491	+	861	WP_013030463.1	IS3 family transposase	-
SLIT_RS13925	2814737..2815060	+	324	WP_013030910.1	type II toxin-antitoxin system RelE/ParE family toxin	-
SLIT_RS13930	2815057..2815389	+	333	WP_013030911.1	putative addiction module antidote protein	-
SLIT_RS13935	2815470..2815883	+	414	WP_013030912.1	DUF1398 family protein	-
SLIT_RS13940	2816000..2816857	-	858	WP_013030913.1	class I SAM-dependent methyltransferase	-
SLIT_RS13945	2817425..2817709	+	285	WP_013030914.1	type II toxin-antitoxin system HigB family toxin	Toxin
SLIT_RS13950	2817706..2818107	+	402	WP_013030915.1	transcriptional regulator	Antitoxin
SLIT_RS13955	2818104..2818325	+	222	WP_150103017.1	hypothetical protein	-
SLIT_RS13960	2818410..2819156	-	747	WP_013030917.1	UPF0149 family protein	-
SLIT_RS13965	2819371..2820042	-	672	WP_041421404.1	dUTP diphosphatase	-
SLIT_RS13970	2820200..2820685	+	486	WP_013030919.1	hypothetical protein	-
SLIT_RS13975	2820740..2821393	-	654	WP_013030920.1	class I SAM-dependent methyltransferase	-
SLIT_RS13980	2821784..2822377	+	594	WP_190272167.1	cyclic nucleotide-binding domain-containing protein	-
SLIT_RS13985	2822395..2822646	-	252	WP_013030922.1	DUF2934 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-90)

Antitoxin

• Antitoxin HigA (13-133)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 10.3177

>T24658 WP_013030914.1 NC_013959:2817425-2817709 [Sideroxydans lithotrophicus ES-1]
MHIITHRRIVTAQQEHPQCASALEQWYRLCKKAEWRNFSELRKLFPSTDKVGDVFVFDIGGNKLRLIAAIHFNTGRVFVR
AVLPHKEYDQGGWK

Nucleotide

• Length: 285 bp

>T24658 NC_013959:2817425-2817709 [Sideroxydans lithotrophicus ES-1]
ATGCACATCATCACTCACCGCCGCATCGTGACTGCGCAGCAAGAGCATCCGCAATGCGCGAGTGCTTTGGAACAGTGGTA
CCGGCTGTGCAAAAAGGCCGAATGGCGCAATTTCTCGGAGTTGCGAAAGCTGTTCCCCAGCACTGACAAGGTGGGCGATG
TATTCGTGTTCGACATTGGCGGAAACAAGCTGCGCCTGATTGCCGCTATCCACTTCAATACGGGACGGGTGTTTGTCAGA
GCGGTATTGCCGCATAAGGAATACGACCAAGGAGGTTGGAAATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14471.52 Da
• Isoelectric Point: 5.2327

>AT24658 WP_013030915.1 NC_013959:2817706-2818107 [Sideroxydans lithotrophicus ES-1]
MNARVKAAIEHWSYVAPLLQPARNAKEYAQLVEALDAAIDAGGADEKHPLARLVDYLGDLVAEYEAKDKMPKAATGADAL
RYLMQCDGLRQVDLPELGSQGVVSELLSGRREFNTRQAKALAERFGVSAALFL

Nucleotide

• Length: 402 bp

>AT24658 NC_013959:2817706-2818107 [Sideroxydans lithotrophicus ES-1]
ATGAACGCACGAGTCAAAGCAGCAATCGAGCATTGGAGCTATGTCGCACCGCTGTTGCAGCCTGCGCGCAATGCCAAGGA
GTATGCGCAGTTGGTTGAGGCGCTGGACGCAGCTATCGATGCAGGCGGTGCGGATGAGAAACATCCGCTCGCGCGTCTGG
TTGATTACCTCGGCGATCTGGTTGCCGAATATGAAGCCAAAGACAAAATGCCGAAGGCTGCGACTGGCGCGGATGCGCTA
CGTTACCTGATGCAGTGCGATGGCTTGCGCCAGGTTGATCTGCCGGAATTGGGTAGCCAGGGCGTGGTATCCGAGTTGCT
TTCTGGGCGGCGCGAGTTCAATACCCGCCAGGCAAAAGCTTTGGCAGAACGGTTTGGTGTTTCGGCGGCATTGTTTTTGT
GA

Structures

Toxin

• AlphaFold DB: D5CPM3

Antitoxin

• AlphaFold DB: D5CPM4