Detailed information of TA system TA024653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/DUF433(antitoxin)
• Location: 681075..681631
• Replicon: chromosome
• Accession: NC_013959
• Organism: Sideroxydans lithotrophicus ES-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: D5CNK3
• Locus tag: SLIT_RS03365
• Protein ID: WP_013028815.1
• Coordinates: 681075..681404 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D5CNK4
• Locus tag: SLIT_RS03370
• Protein ID: WP_013028816.1
• Coordinates: 681407..681631 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SLIT_RS03345	676299..677336	-	1038	WP_013028811.1	tetratricopeptide repeat protein	-
SLIT_RS03350	677487..679166	+	1680	WP_013028812.1	glutamine--tRNA ligase/YqeY domain fusion protein	-
SLIT_RS03355	679163..679513	+	351	WP_013028813.1	membrane protein	-
SLIT_RS03360	679521..680996	+	1476	WP_013028814.1	cysteine--tRNA ligase	-
SLIT_RS03365	681075..681404	-	330	WP_013028815.1	DUF5615 family PIN-like protein	Toxin
SLIT_RS03370	681407..681631	-	225	WP_013028816.1	DUF433 domain-containing protein	Antitoxin
SLIT_RS03375	681830..682507	-	678	WP_013028817.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
SLIT_RS03380	682504..684291	-	1788	WP_013028818.1	hypothetical protein	-
SLIT_RS03385	684306..684674	-	369	WP_013028819.1	hypothetical protein	-
SLIT_RS03390	684671..685267	-	597	WP_013028820.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	671609..681631	10022

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Domain of unknown function DUF5615 (1-77)

Antitoxin

• Protein of unknown function DUF433 (7-62)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11965.78 Da
• Isoelectric Point: 4.7584

>T24653 WP_013028815.1 NC_013959:c681404-681075 [Sideroxydans lithotrophicus ES-1]
MRFLVDAQLPPALARYLSSAGHQAEHVYDIGLAAASDRIIWNYAVSNHAAIFTKDEDFVSMVGTSADAPPIVWVRVGNIG
KQALLTWIEPMMARIISEIEAGEKLIEIT

Nucleotide

• Length: 330 bp

>T24653 NC_013959:c681404-681075 [Sideroxydans lithotrophicus ES-1]
ATGAGATTTCTTGTCGATGCGCAGCTTCCGCCCGCGCTGGCGCGCTACCTCAGTAGCGCAGGACATCAGGCAGAGCACGT
CTATGACATAGGATTGGCGGCAGCAAGCGACAGGATCATCTGGAACTACGCCGTCTCAAATCACGCTGCCATCTTCACCA
AAGACGAGGACTTCGTCAGCATGGTCGGCACCAGTGCGGACGCGCCGCCGATAGTTTGGGTACGCGTTGGAAATATTGGC
AAACAGGCCTTGCTGACCTGGATAGAACCGATGATGGCGCGAATCATCTCCGAGATCGAAGCAGGAGAAAAGCTGATTGA
GATTACCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8279.65 Da
• Isoelectric Point: 4.6854

>AT24653 WP_013028816.1 NC_013959:c681631-681407 [Sideroxydans lithotrophicus ES-1]
MGQVTRITVDPNMCGGRPCIRGLRVRVKDVLEMLASGMTREEILKDFPYLETEDITATLEYAAMQVDHPILQAA

Nucleotide

• Length: 225 bp

>AT24653 NC_013959:c681631-681407 [Sideroxydans lithotrophicus ES-1]
ATGGGACAAGTCACCAGAATCACCGTTGACCCGAATATGTGCGGTGGCAGGCCTTGTATTCGCGGTCTACGGGTACGTGT
AAAAGACGTACTGGAAATGCTGGCATCCGGCATGACCAGAGAAGAAATTCTCAAAGATTTCCCTTATCTTGAAACCGAGG
ATATTACTGCCACACTTGAATATGCTGCCATGCAAGTAGACCACCCAATACTGCAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: D5CNK3

Antitoxin

• AlphaFold DB: D5CNK4