Detailed information of TA system TA246516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 988640..989975
• Replicon: chromosome
• Accession: NZ_CP097896
• Organism: Pectobacterium actinidiae strain GX-Pa1

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: M9782_RS04430
• Protein ID: WP_261850156.1
• Coordinates: 989028..989975 (+)
• Length: 316 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: -
• Locus tag: M9782_RS04425
• Protein ID: WP_261850157.1
• Coordinates: 988640..989035 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M9782_RS04390 (M9782_04390)	984545..985099	+	555	WP_275071732.1	hypothetical protein	-
M9782_RS04395 (M9782_04395)	985128..985733	+	606	WP_261850135.1	hypothetical protein	-
M9782_RS04400 (M9782_04400)	985940..986413	+	474	WP_261850134.1	DNA repair protein RadC	-
M9782_RS04405 (M9782_04405)	986438..986774	+	337	Protein_849	type IV toxin-antitoxin system YeeU family antitoxin	-
M9782_RS04410 (M9782_04410)	986844..987164	+	321	WP_275071733.1	TA system toxin CbtA family protein	-
M9782_RS04415 (M9782_04415)	987282..988115	+	834	WP_261850159.1	DUF4942 domain-containing protein	-
M9782_RS04420 (M9782_04420)	988225..988467	+	243	WP_261850158.1	ribbon-helix-helix domain-containing protein	-
M9782_RS04425 (M9782_04425)	988640..989035	+	396	WP_261850157.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
M9782_RS04430 (M9782_04430)	989028..989975	+	948	WP_261850156.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
M9782_RS04435 (M9782_04435)	990365..991228	+	864	Protein_855	integrase arm-type DNA-binding domain-containing protein	-
M9782_RS04440 (M9782_04440)	991295..991426	+	132	Protein_856	CopG family transcriptional regulator	-
M9782_RS04445 (M9782_04445)	991732..992037	+	306	WP_015848020.1	helix-turn-helix transcriptional regulator	-
M9782_RS04450 (M9782_04450)	992042..993376	+	1335	WP_275071734.1	type II toxin-antitoxin system HipA family toxin	-
M9782_RS04455 (M9782_04455)	993653..993775	-	123	Protein_859	transcriptional regulator	-
M9782_RS04460 (M9782_04460)	994049..994477	+	429	WP_275071735.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	960820..994477	33657

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (36-303)

Antitoxin

• Protein of unknown function DUF6088 (7-125)

Sequences

Toxin

Protein

• Length: 316 a.a.
• Molecular weight: 35766.01 Da
• Isoelectric Point: 4.6633

>T246516 WP_261850156.1 NZ_CP097896:989028-989975 [Pectobacterium actinidiae]
MDDLTKLFPDVADALGIESVAIVEKDYYIVELLRLLQPLAFDTHQLVFAGGTALSKAGISLNRMSEDVDIKLVPVAGFSL
QYSRNQRKNIRKDIIKTITETIVASGMFSMDEEYPKVTRDENRYNEIPVRYPQKYAQAPCLRPFIKLELMETDLLEAAVN
RDISSLVIDLTKKGEVVSAFPCASIDSTQAEKLISMMRRTAASLRDVERAVDESLVRHIYDNFCIVREKGADVPVLKRFV
QECIKQDIERYGNQYPAFSEAPVEELKMGLEELGSNSVYEVRYQQFVTPMVFGGTQISWEEAYSCFRQTALEVLQ

Nucleotide

• Length: 948 bp

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14398.67 Da
• Isoelectric Point: 10.9292

>AT246516 WP_261850157.1 NZ_CP097896:988640-989035 [Pectobacterium actinidiae]
MTTKARIQSRLKRSKRYVFTRDDFKDLAGYDQVGRALRELVKEGLLLKVGYGVYTKARKNVITGRLMPASPGGSAAVVIE
ALERLKVDYQLADAASAYNSGKSTQVPASMEIKTSPRFKRTLSVGNSKLNG

Nucleotide

• Length: 396 bp