246512

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1271658..1272575 Replicon chromosome
Accession NZ_CP097895
Organism Bacillus velezensis strain B1

Toxin (Protein)


Gene name spoIISA Uniprot ID A0A6A8LGT8
Locus tag M8561_RS06515 Protein ID WP_003154806.1
Coordinates 1271829..1272575 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag M8561_RS06510 Protein ID WP_003154807.1
Coordinates 1271658..1271828 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8561_RS06470 (M8561_06470) 1266890..1268512 + 1623 WP_024085192.1 pyocin knob domain-containing protein -
M8561_RS06475 (M8561_06475) 1268525..1268896 + 372 WP_024085193.1 XkdW family protein -
M8561_RS06480 (M8561_06480) 1268902..1269099 + 198 WP_003154819.1 XkdX family protein -
M8561_RS06485 (M8561_06485) 1269156..1269917 + 762 WP_024085194.1 hypothetical protein -
M8561_RS06490 (M8561_06490) 1269969..1270232 + 264 WP_003154815.1 hemolysin XhlA family protein -
M8561_RS06495 (M8561_06495) 1270246..1270509 + 264 WP_003154813.1 phage holin -
M8561_RS06500 (M8561_06500) 1270523..1271401 + 879 WP_024085195.1 N-acetylmuramoyl-L-alanine amidase -
M8561_RS06505 (M8561_06505) 1271436..1271561 - 126 WP_003154809.1 hypothetical protein -
M8561_RS06510 (M8561_06510) 1271658..1271828 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
M8561_RS06515 (M8561_06515) 1271829..1272575 - 747 WP_003154806.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
M8561_RS06520 (M8561_06520) 1272680..1273678 - 999 WP_003154805.1 inorganic phosphate transporter -
M8561_RS06525 (M8561_06525) 1273691..1274308 - 618 WP_003154804.1 DUF47 domain-containing protein -
M8561_RS06530 (M8561_06530) 1274594..1275910 - 1317 WP_003154801.1 amino acid permease -
M8561_RS06535 (M8561_06535) 1276234..1277184 + 951 WP_024085196.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1227867..1329934 102067


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29034.50 Da        Isoelectric Point: 4.6947

>T246512 WP_003154806.1 NZ_CP097895:c1272575-1271829 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT246512 WP_003154807.1 NZ_CP097895:c1271828-1271658 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6A8LGT8

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References