Detailed information of TA system TA024650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2590156..2590746
• Replicon: chromosome
• Accession: NC_013956
• Organism: Pantoea ananatis LMG 20103

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: PANA_RS11815
• Protein ID: WP_041457686.1
• Coordinates: 2590156..2590488 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: PANA_RS11820
• Protein ID: WP_041457688.1
• Coordinates: 2590489..2590746 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PANA_RS11795	2586715..2586969	-	255	WP_013026202.1	Arc family DNA-binding protein	-
PANA_RS11800	2587043..2587747	-	705	WP_013026203.1	phosphohydrolase	-
PANA_RS11805	2588360..2589037	+	678	WP_013026204.1	hypothetical protein	-
PANA_RS11810	2589143..2589976	+	834	WP_013026205.1	DgsA anti-repressor MtfA	-
PANA_RS11815	2590156..2590488	-	333	WP_041457686.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PANA_RS11820	2590489..2590746	-	258	WP_041457688.1	antitoxin	Antitoxin
PANA_RS21825	2591094..2591299	-	206	Protein_2318	helix-turn-helix domain-containing protein	-
PANA_RS11830	2591801..2592763	-	963	WP_014605551.1	ferrochelatase	-
PANA_RS11840	2593512..2594750	+	1239	WP_041457689.1	MFS transporter	-
PANA_RS21835	2595038..2595463	-	426	WP_014605549.1	pentapeptide repeat-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

• Antitoxin PemI-like (4-83)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11945.66 Da
• Isoelectric Point: 6.9807

>T24650 WP_041457686.1 NC_013956:c2590488-2590156 [Pantoea ananatis LMG 20103]
MERGEIWLVSLDPNAGHEQSGRRPVLIVSPASFNQFTRLPVVVPVTNGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTL
DMEARNGKYLERIPEVIVNEVVARLEAILS

Nucleotide

• Length: 333 bp

>T24650 NC_013956:c2590488-2590156 [Pantoea ananatis LMG 20103]
ATGGAAAGAGGGGAAATCTGGCTCGTTTCACTAGATCCGAATGCCGGGCATGAACAAAGCGGAAGGCGTCCAGTACTGAT
TGTTTCTCCGGCGTCCTTTAATCAGTTTACGCGGCTACCTGTGGTTGTTCCCGTGACCAACGGCGGAAACTTTGCCCGCG
CAGCGGGGTTTACTGTTTCACTGGATGGCGCAGGAACGAAAACAACGGGGGTAATTCGTTGTGACCAGCCCAGAACCCTT
GATATGGAAGCCCGGAACGGCAAATATCTTGAACGAATACCTGAAGTTATTGTGAATGAAGTGGTGGCCAGACTGGAGGC
CATTCTGAGCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9281.74 Da
• Isoelectric Point: 4.5821

>AT24650 WP_041457688.1 NC_013956:c2590746-2590489 [Pantoea ananatis LMG 20103]
MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDSGCLIIEPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAV
GRELL

Nucleotide

• Length: 258 bp

>AT24650 NC_013956:c2590746-2590489 [Pantoea ananatis LMG 20103]
ATGTATACCACTCGTCTGAAAAAGGTTGGCGGATCTGTCATGCTGGCGGTCCCTCCCGCCGTGCTGAAAACGCTGGGGCT
GTCGACGGACAGCGAAGTGGGCATGACCATTGATAGTGGCTGCCTCATAATTGAACCTCAAAAACGTCCCCGCTATTCAC
TCGAAGAACTACTGGCGCAGTGCGATCCGCACGCTGAAATTAGCGAAGAGGGTCGGGAATGGATTGATGCGCCTGCGGTG
GGCAGGGAGCTCCTGTAG