Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 2416281..2417112 | Replicon | chromosome |
| Accession | NZ_CP097884 | ||
| Organism | Escherichia coli str. K-12 substr. MG1655 strain K-12 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | S1P968 |
| Locus tag | NAG72_RS11460 | Protein ID | WP_000854814.1 |
| Coordinates | 2416281..2416655 (-) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | P76364 |
| Locus tag | NAG72_RS11465 | Protein ID | WP_001285584.1 |
| Coordinates | 2416744..2417112 (-) | Length | 123 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| NAG72_RS11420 (2411677) | 2411677..2412843 | + | 1167 | WP_000830156.1 | serine-type D-Ala-D-Ala carboxypeptidase DacD | - |
| NAG72_RS11425 (2412962) | 2412962..2413435 | + | 474 | WP_001105415.1 | DNA gyrase inhibitor SbmC | - |
| NAG72_RS11430 (2413633) | 2413633..2414691 | + | 1059 | WP_001200891.1 | FUSC family protein | - |
| NAG72_RS11435 (2414863) | 2414863..2415192 | + | 330 | WP_000450409.1 | DUF496 family protein | - |
| NAG72_RS11440 (2415293) | 2415293..2415427 | - | 135 | WP_001297944.1 | EutP/PduV family microcompartment system protein | - |
| NAG72_RS11445 (2415547) | 2415547..2415675 | + | 129 | Protein_2216 | transposase domain-containing protein | - |
| NAG72_RS11450 (2415964) | 2415964..2416044 | - | 81 | Protein_2217 | hypothetical protein | - |
| NAG72_RS11455 (2416090) | 2416090..2416284 | - | 195 | WP_000988600.1 | DUF5983 family protein | - |
| NAG72_RS11460 (2416281) | 2416281..2416655 | - | 375 | WP_000854814.1 | type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA | Toxin |
| NAG72_RS11465 (2416744) | 2416744..2417112 | - | 369 | WP_001285584.1 | type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA | Antitoxin |
| NAG72_RS11470 (2417186) | 2417186..2417407 | - | 222 | WP_000692323.1 | DUF987 domain-containing protein | - |
| NAG72_RS11475 (2417470) | 2417470..2417916 | - | 447 | WP_000187523.1 | RadC family protein | - |
| NAG72_RS11480 (2417913) | 2417913..2419445 | - | 1533 | WP_001350525.1 | protein YeeR | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 13898.94 Da Isoelectric Point: 7.8522
>T246495 WP_000854814.1 NZ_CP097884:c2416655-2416281 [Escherichia coli str. K-12 substr. MG1655]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
Download Length: 375 bp
Antitoxin
Download Length: 123 a.a. Molecular weight: 13651.52 Da Isoelectric Point: 5.9541
>AT246495 WP_001285584.1 NZ_CP097884:c2417112-2416744 [Escherichia coli str. K-12 substr. MG1655]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN
Download Length: 369 bp
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829FY50 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| PDB | 2H28 | |
| AlphaFold DB | A0A1M2E8G6 |