Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1467794..1468448 Replicon chromosome
Accession NZ_CP097884
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag NAG72_RS07035 Protein ID WP_000244777.1
Coordinates 1468041..1468448 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NAG72_RS07030 Protein ID WP_000354046.1
Coordinates 1467794..1468060 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NAG72_RS07005 (1462963) 1462963..1463706 + 744 WP_000951964.1 SDR family oxidoreductase -
NAG72_RS07010 (1463763) 1463763..1465196 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
NAG72_RS07015 (1465241) 1465241..1465552 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
NAG72_RS07020 (1465716) 1465716..1466375 + 660 WP_000250274.1 hemolysin III family protein -
NAG72_RS07025 (1466571) 1466571..1467551 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
NAG72_RS07030 (1467794) 1467794..1468060 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NAG72_RS07035 (1468041) 1468041..1468448 + 408 WP_000244777.1 protein YgfX Toxin
NAG72_RS07040 (1468488) 1468488..1469009 - 522 WP_001055874.1 flavodoxin FldB -
NAG72_RS07045 (1469121) 1469121..1470017 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NAG72_RS07050 (1470042) 1470042..1470752 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NAG72_RS07055 (1470758) 1470758..1472491 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T246490 WP_000244777.1 NZ_CP097884:1468041-1468448 [Escherichia coli str. K-12 substr. MG1655]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT246490 WP_000354046.1 NZ_CP097884:1467794-1468060 [Escherichia coli str. K-12 substr. MG1655]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References