Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 154559..155056 Replicon chromosome
Accession NZ_CP097884
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NAG72_RS00780 Protein ID WP_001173343.1
Coordinates 154559..154732 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NAG72_RS00785 Protein ID WP_000398619.1
Coordinates 154760..155056 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NAG72_RS00770 (151229) 151229..152686 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
NAG72_RS00775 (152923) 152923..154440 + 1518 WP_001295074.1 lysine--tRNA ligase -
NAG72_RS00780 (154559) 154559..154732 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NAG72_RS00785 (154760) 154760..155056 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NAG72_RS00790 (155283) 155283..155555 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
NAG72_RS00795 (155567) 155567..155797 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
NAG72_RS00800 (155978) 155978..157609 + 1632 WP_001216477.1 sensor histidine kinase -
NAG72_RS00805 (157606) 157606..158325 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T246486 WP_001173343.1 NZ_CP097884:c154732-154559 [Escherichia coli str. K-12 substr. MG1655]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT246486 WP_000398619.1 NZ_CP097884:c155056-154760 [Escherichia coli str. K-12 substr. MG1655]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References