Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4204613..4205110 Replicon chromosome
Accession NZ_CP097882
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NAG70_RS20615 Protein ID WP_001173343.1
Coordinates 4204613..4204786 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NAG70_RS20620 Protein ID WP_000398619.1
Coordinates 4204814..4205110 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NAG70_RS20605 (4201283) 4201283..4202740 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
NAG70_RS20610 (4202977) 4202977..4204494 + 1518 WP_001295074.1 lysine--tRNA ligase -
NAG70_RS20615 (4204613) 4204613..4204786 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NAG70_RS20620 (4204814) 4204814..4205110 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NAG70_RS20625 (4205337) 4205337..4205609 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
NAG70_RS20630 (4205621) 4205621..4205851 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
NAG70_RS20635 (4206032) 4206032..4207663 + 1632 WP_001216477.1 sensor histidine kinase -
NAG70_RS20640 (4207660) 4207660..4208379 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T246458 WP_001173343.1 NZ_CP097882:c4204786-4204613 [Escherichia coli str. K-12 substr. MG1655]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT246458 WP_000398619.1 NZ_CP097882:c4205110-4204814 [Escherichia coli str. K-12 substr. MG1655]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References