Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4133814..4134311 Replicon chromosome
Accession NZ_CP097881
Organism Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NAG69_RS20145 Protein ID WP_001173343.1
Coordinates 4133814..4133987 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NAG69_RS20150 Protein ID WP_000398619.1
Coordinates 4134015..4134311 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NAG69_RS20135 (4130484) 4130484..4131941 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
NAG69_RS20140 (4132178) 4132178..4133695 + 1518 WP_001295074.1 lysine--tRNA ligase -
NAG69_RS20145 (4133814) 4133814..4133987 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NAG69_RS20150 (4134015) 4134015..4134311 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NAG69_RS20155 (4134538) 4134538..4134810 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
NAG69_RS20160 (4134822) 4134822..4135052 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
NAG69_RS20165 (4135233) 4135233..4136864 + 1632 WP_001216477.1 sensor histidine kinase -
NAG69_RS20170 (4136861) 4136861..4137580 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T246432 WP_001173343.1 NZ_CP097881:c4133987-4133814 [Escherichia coli str. K-12 substr. MG1655]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT246432 WP_000398619.1 NZ_CP097881:c4134311-4134015 [Escherichia coli str. K-12 substr. MG1655]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References