Detailed information of TA system TA024639

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1166711..1167328
• Replicon: chromosome
• Accession: NC_013956
• Organism: Pantoea ananatis LMG 20103

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: D4GLR0
• Locus tag: PANA_RS05205
• Protein ID: WP_013024903.1
• Coordinates: 1166711..1166926 (-)
• Length: 72 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A8A4KA49
• Locus tag: PANA_RS05210
• Protein ID: WP_015700827.1
• Coordinates: 1166951..1167328 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PANA_RS05180	1162632..1162970	+	339	WP_013024898.1	P-II family nitrogen regulator	-
PANA_RS05185	1163004..1164293	+	1290	WP_014606435.1	ammonium transporter AmtB	-
PANA_RS05190	1164340..1165203	-	864	WP_013024900.1	acyl-CoA thioesterase II	-
PANA_RS05195	1165396..1165953	+	558	WP_013024901.1	YbaY family lipoprotein	-
PANA_RS05200	1166002..1166313	-	312	WP_015700828.1	MGMT family protein	-
PANA_RS05205	1166711..1166926	-	216	WP_013024903.1	hemolysin expression modulator Hha	Toxin
PANA_RS05210	1166951..1167328	-	378	WP_015700827.1	Hha toxicity modulator TomB	Antitoxin
PANA_RS05215	1167475..1167828	-	354	WP_013024905.1	hypothetical protein	-
PANA_RS05220	1168183..1168851	+	669	WP_014606429.1	ABC transporter ATP-binding protein	-
PANA_RS05225	1168848..1169687	+	840	WP_013024907.1	metal ABC transporter permease	-
PANA_RS05230	1169700..1170578	+	879	WP_013024908.1	metal ABC transporter substrate-binding protein	-
PANA_RS05235	1170638..1170778	-	141	WP_014606428.1	type B 50S ribosomal protein L36	-
PANA_RS05240	1170790..1171044	-	255	WP_014606427.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (12-68)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 72 a.a.
• Molecular weight: 8552.98 Da
• Isoelectric Point: 9.4825

>T24639 WP_013024903.1 NC_013956:c1166926-1166711 [Pantoea ananatis LMG 20103]
MNKTLTKTDYLMRLRRCRSIDTLERVIEKNKYELPDEELAVFYSAADHRLAELTMNKLYDKVPVSVWKFVR

Nucleotide

• Length: 216 bp

>T24639 NC_013956:c1166926-1166711 [Pantoea ananatis LMG 20103]
ATGAACAAAACCCTAACTAAAACTGATTATTTGATGCGACTGAGGCGCTGTCGTTCTATTGACACGCTGGAACGCGTGAT
TGAAAAAAATAAGTACGAATTACCCGATGAAGAATTAGCAGTATTTTACTCAGCAGCCGATCACCGCCTGGCTGAACTGA
CGATGAACAAGCTCTATGACAAAGTCCCGGTGTCAGTCTGGAAATTTGTACGCTAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 14722.35 Da
• Isoelectric Point: 4.3976

>AT24639 WP_015700827.1 NC_013956:c1167328-1166951 [Pantoea ananatis LMG 20103]
MDEYSPKRHDIAQLKYLCENLFDDSMATLTDSHHGWVNDPTAESNLQLNDLIEHIASFTMNYKIKHVEDEALITQIDEYL
DDTFMLFSNYSVNTQDLQRWQRSAKRLFNVFTEECAFLQQPSHSL

Nucleotide

• Length: 378 bp

>AT24639 NC_013956:c1167328-1166951 [Pantoea ananatis LMG 20103]
ATGGACGAATACTCACCGAAACGGCATGATATTGCCCAGCTTAAATACTTGTGTGAGAATCTGTTTGACGACAGCATGGC
GACATTAACTGACAGTCATCACGGCTGGGTTAATGACCCCACTGCCGAAAGCAATTTACAGCTAAACGATTTGATTGAGC
ACATCGCCTCTTTCACCATGAATTACAAAATTAAGCACGTTGAAGACGAAGCACTGATTACCCAAATCGATGAATATCTT
GATGATACCTTTATGCTGTTTAGCAACTACAGTGTAAATACTCAGGATCTTCAGCGCTGGCAACGCTCAGCGAAACGTTT
ATTTAATGTGTTTACTGAAGAGTGCGCTTTTCTTCAACAGCCAAGCCATTCATTATAG