Detailed information of TA system TA024636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 4368932..4369560
• Replicon: chromosome
• Accession: NC_013947
• Organism: Stackebrandtia nassauensis DSM 44728

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: D3Q0Z4
• Locus tag: SNAS_RS20190
• Protein ID: WP_013019315.1
• Coordinates: 4368932..4369345 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: D3Q0Z5
• Locus tag: SNAS_RS20195
• Protein ID: WP_013019316.1
• Coordinates: 4369342..4369560 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SNAS_RS20160	4364250..4365062	-	813	WP_211207207.1	hypothetical protein	-
SNAS_RS33030	4365298..4366371	+	1074	WP_013019310.1	family 43 glycosylhydrolase	-
SNAS_RS34235	4366449..4366622	-	174	WP_013019311.1	DUF397 domain-containing protein	-
SNAS_RS20170	4366612..4367442	-	831	WP_013019312.1	helix-turn-helix domain-containing protein	-
SNAS_RS20175	4367620..4367898	+	279	WP_013019313.1	hypothetical protein	-
SNAS_RS34240	4367910..4368305	+	396	Protein_4070	response regulator transcription factor	-
SNAS_RS20185	4368309..4368899	-	591	WP_169313907.1	SMI1/KNR4 family protein	-
SNAS_RS20190	4368932..4369345	-	414	WP_013019315.1	PIN domain-containing protein	Toxin
SNAS_RS20195	4369342..4369560	-	219	WP_013019316.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
SNAS_RS20200	4369803..4370393	+	591	WP_013019317.1	helix-turn-helix domain-containing protein	-
SNAS_RS20205	4370390..4371046	+	657	WP_013019318.1	SRPBCC domain-containing protein	-
SNAS_RS20210	4371162..4371764	+	603	WP_013019319.1	GNAT family N-acetyltransferase	-
SNAS_RS20215	4371771..4372169	+	399	WP_013019320.1	hypothetical protein	-
SNAS_RS20220	4372185..4373006	+	822	WP_013019321.1	APH(3'') family aminoglycoside O-phosphotransferase	-
SNAS_RS34245	4373045..4373638	+	594	WP_013019322.1	alpha-ketoglutarate-dependent dioxygenase AlkB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-45)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15466.50 Da
• Isoelectric Point: 6.8627

>T24636 WP_013019315.1 NC_013947:c4369345-4368932 [Stackebrandtia nassauensis DSM 44728]
MIRYLVDSSALWRILRDGGVRAAWQEAISNGAVGSCQPQRCEFKRSARDRDEFDAMSRMFEDLYPDVAVHKSAWKWMDSV
QYRLSAKGKHRGLSVVDLVVAATAAYHGLIVLHDDNDFATVVTEAPDLSERNVNRLP

Nucleotide

• Length: 414 bp

>T24636 NC_013947:c4369345-4368932 [Stackebrandtia nassauensis DSM 44728]
ATGATTCGGTACCTGGTGGATTCCTCGGCACTGTGGAGGATCTTGCGGGATGGGGGAGTACGGGCCGCCTGGCAGGAGGC
GATCAGCAACGGCGCGGTGGGTTCGTGCCAGCCGCAGCGTTGTGAGTTCAAGCGGTCGGCTCGGGATCGGGACGAGTTCG
ACGCGATGAGCCGGATGTTCGAGGACTTGTATCCGGATGTCGCCGTTCACAAGTCGGCGTGGAAGTGGATGGATTCGGTT
CAGTATCGGTTGTCGGCCAAGGGGAAGCACCGGGGATTGTCGGTTGTGGATCTCGTGGTGGCGGCGACGGCGGCATATCA
CGGGCTGATCGTTCTGCATGACGACAATGACTTCGCGACGGTCGTCACCGAGGCCCCGGACCTGAGTGAGCGCAACGTGA
ATCGGTTGCCGTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8396.36 Da
• Isoelectric Point: 5.1529

>AT24636 WP_013019316.1 NC_013947:c4369560-4369342 [Stackebrandtia nassauensis DSM 44728]
MSVTSLNIDEGALAAVLRLSGVRTKRDAVNLALREYAERHERIAALERYADMAQEWDHESWREQHEMEKRGE

Nucleotide

• Length: 219 bp

>AT24636 NC_013947:c4369560-4369342 [Stackebrandtia nassauensis DSM 44728]
ATGAGCGTGACGTCGTTGAACATAGATGAGGGTGCGCTGGCCGCCGTCTTGCGATTGTCGGGGGTGCGAACCAAGCGGGA
CGCCGTGAACCTGGCGTTGCGGGAGTATGCCGAGCGGCATGAGCGCATCGCAGCCTTGGAGCGGTACGCGGACATGGCTC
AGGAGTGGGACCACGAGTCGTGGCGGGAACAGCACGAGATGGAGAAGCGCGGGGAATGA

Structures

Toxin

• AlphaFold DB: D3Q0Z4

Antitoxin

• AlphaFold DB: D3Q0Z5