Detailed information of TA system TA024629

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 58500..59035
• Replicon: plasmid pO55
• Accession: NC_013942
• Organism: Escherichia coli O55:H7 str. CB9615

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: G2583_RS27580
• Protein ID: WP_001270421.1
• Coordinates: 58500..58787 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1V0M724
• Locus tag: G2583_RS27585
• Protein ID: WP_001132893.1
• Coordinates: 58784..59035 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G2583_RS27550	53903..54082	-	180	WP_000526857.1	hypothetical protein	-
G2583_RS27555	54285..54659	+	375	WP_001368887.1	hypothetical protein	-
G2583_RS31490	54970..55131	-	162	Protein_68	epoxyqueuosine reductase QueH	-
G2583_RS27830	55182..55322	+	141	Protein_69	serine/threonine protein kinase	-
G2583_RS27565	55446..56186	+	741	WP_001066926.1	tyrosine-type recombinase/integrase	-
G2583_RS27570	56470..57447	-	978	WP_000361616.1	RepB family plasmid replication initiator protein	-
G2583_RS29950	58093..58498	-	406	Protein_72	DDE-type integrase/transposase/recombinase	-
G2583_RS27580	58500..58787	-	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G2583_RS27585	58784..59035	-	252	WP_001132893.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
G2583_RS27590	59424..60629	+	1206	WP_000817637.1	AAA family ATPase	-
G2583_RS27595	60626..61588	+	963	WP_000756329.1	ParB/RepB/Spo0J family partition protein	-
G2583_RS27600	61729..62740	-	1012	Protein_77	DUF4113 domain-containing protein	-
G2583_RS27605	62771..63187	+	417	Protein_78	DUF1281 domain-containing protein	-
G2583_RS30745	63191..63496	+	306	WP_032325408.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	stcE / exeE / exeG / nleA/espI	1..66001	66001

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T24629 WP_001270421.1 NC_013942:c58787-58500 [Escherichia coli O55:H7 str. CB9615]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T24629 NC_013942:c58787-58500 [Escherichia coli O55:H7 str. CB9615]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAG
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9192.50 Da
• Isoelectric Point: 4.2108

>AT24629 WP_001132893.1 NC_013942:c59035-58784 [Escherichia coli O55:H7 str. CB9615]
MPNIILSDTSASVSELKKNPMATVSAGDGFPIAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT24629 NC_013942:c59035-58784 [Escherichia coli O55:H7 str. CB9615]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGATCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTAGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A1V0M724