Detailed information of TA system TA246280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1635946..1636577
• Replicon: chromosome
• Accession: NZ_CP097828
• Organism: Shigella flexneri strain E

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: M9383_RS08295
• Protein ID: WP_025760846.1
• Coordinates: 1636302..1636577 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U9Y7E1
• Locus tag: M9383_RS08290
• Protein ID: WP_000593555.1
• Coordinates: 1635946..1636305 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M9383_RS08265 (1632078)	1632078..1633022	+	945	WP_000947076.1	nickel ABC transporter permease subunit NikB	-
M9383_RS08270 (1633019)	1633019..1633852	+	834	WP_001008974.1	nickel ABC transporter permease subunit NikC	-
M9383_RS08275 (1633852)	1633852..1634616	+	765	WP_001136248.1	nickel import ATP-binding protein NikD	-
M9383_RS08280 (1634613)	1634613..1635419	+	807	WP_000173660.1	nickel import ATP-binding protein NikE	-
M9383_RS08285 (1635425)	1635425..1635826	+	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
M9383_RS08290 (1635946)	1635946..1636305	-	360	WP_000593555.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
M9383_RS08295 (1636302)	1636302..1636577	-	276	WP_025760846.1	type II toxin-antitoxin system HicA family toxin	Toxin
M9383_RS08300 (1636636)	1636636..1637760	-	1125	WP_001216257.1	ABC transporter permease	-
M9383_RS08305 (1637760)	1637760..1640495	-	2736	WP_000149172.1	ribosome-associated ATPase/putative transporter RbbA	-
M9383_RS08310 (1640492)	1640492..1641559	-	1068	WP_000361470.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-81)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10145.95 Da
• Isoelectric Point: 10.6469

>T246280 WP_025760846.1 NZ_CP097828:c1636577-1636302 [Shigella flexneri]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKKGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
CDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T246280 NZ_CP097828:c1636577-1636302 [Shigella flexneri]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGAAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
TGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT246280 WP_000593555.1 NZ_CP097828:c1636305-1635946 [Shigella flexneri]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT246280 NZ_CP097828:c1636305-1635946 [Shigella flexneri]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LKZ6