Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 31553..32100 Replicon plasmid pO55
Accession NC_013942
Organism Escherichia coli O55:H7 str. CB9615

Toxin (Protein)


Gene name parE Uniprot ID A0A896ZGX1
Locus tag G2583_RS27440 Protein ID WP_000969997.1
Coordinates 31819..32100 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A7U8WKC6
Locus tag G2583_RS27435 Protein ID WP_000079946.1
Coordinates 31553..31822 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G2583_RS27400 27254..27769 + 516 WP_000998852.1 type II secretion system protein M -
G2583_RS27405 27821..28654 + 834 WP_000088803.1 A24 family peptidase -
G2583_RS27410 28748..29149 + 402 WP_001222321.1 GspS family T2SS pilot lipoprotein variant EptO -
G2583_RS30720 29224..29439 + 216 Protein_38 IS3 family transposase -
G2583_RS31480 29738..30031 + 294 Protein_39 hypothetical protein -
G2583_RS27425 30260..30508 + 249 WP_000083841.1 replication regulatory protein RepA -
G2583_RS31655 30531..30638 + 108 Protein_41 incFII family plasmid replication initiator RepA -
G2583_RS31660 31340..31498 + 159 WP_000829588.1 hypothetical protein -
G2583_RS27435 31553..31822 + 270 WP_000079946.1 hypothetical protein Antitoxin
G2583_RS27440 31819..32100 + 282 WP_000969997.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
G2583_RS27445 32296..32637 - 342 WP_000578011.1 hypothetical protein -
G2583_RS27450 32937..33524 + 588 WP_001418189.1 serine acetyltransferase -
G2583_RS27455 33806..34399 - 594 WP_000517696.1 hypothetical protein -
G2583_RS27460 34753..36099 + 1347 WP_001132032.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - stcE / exeE / exeG / nleA/espI 1..66001 66001


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10879.54 Da        Isoelectric Point: 8.4615

>T24628 WP_000969997.1 NC_013942:31819-32100 [Escherichia coli O55:H7 str. CB9615]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YVLRVLHTAQKWP

Download         Length: 282 bp

>T24628 NC_013942:31819-32100 [Escherichia coli O55:H7 str. CB9615]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAACGGTC
GTGTGGAAGGAACCCGGGAACTGGTTATTCACCCCCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATGTCCTGCGCGTGTTGCATACCGCACAGAAGTGGCCATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10210.55 Da        Isoelectric Point: 8.8735

>AT24628 WP_000079946.1 NC_013942:31553-31822 [Escherichia coli O55:H7 str. CB9615]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLGRGDVLPHDMVESSATAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT24628 NC_013942:31553-31822 [Escherichia coli O55:H7 str. CB9615]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGGGCGTGGTGATGTGCTTCCACATGACATGGTCGAATCCTCCGCGACTGCATGGCGGGAT
GAAATGAGCAGAAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A896ZGX1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U8WKC6

References