Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5356717..5357342 Replicon chromosome
Accession NZ_CP097808
Organism Klebsiella variicola strain 17YN56

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag M9O80_RS25870 Protein ID WP_008807903.1
Coordinates 5356717..5357100 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M9O80_RS25875 Protein ID WP_004150355.1
Coordinates 5357100..5357342 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M9O80_RS25855 (M9O80_25855) 5354084..5354986 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
M9O80_RS25860 (M9O80_25860) 5354983..5355618 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
M9O80_RS25865 (M9O80_25865) 5355615..5356544 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
M9O80_RS25870 (M9O80_25870) 5356717..5357100 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
M9O80_RS25875 (M9O80_25875) 5357100..5357342 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M9O80_RS25880 (M9O80_25880) 5357547..5358464 + 918 WP_032756518.1 alpha/beta hydrolase -
M9O80_RS25885 (M9O80_25885) 5358479..5359420 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
M9O80_RS25890 (M9O80_25890) 5359465..5359902 - 438 WP_012543288.1 D-aminoacyl-tRNA deacylase -
M9O80_RS25895 (M9O80_25895) 5359899..5360759 - 861 WP_008807907.1 virulence factor BrkB family protein -
M9O80_RS25900 (M9O80_25900) 5360753..5361352 - 600 WP_064176466.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T246275 WP_008807903.1 NZ_CP097808:c5357100-5356717 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT246275 WP_004150355.1 NZ_CP097808:c5357342-5357100 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References