Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1778475..1779065 Replicon chromosome
Accession NZ_CP097808
Organism Klebsiella variicola strain 17YN56

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag M9O80_RS08515 Protein ID WP_008804165.1
Coordinates 1778733..1779065 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag M9O80_RS08510 Protein ID WP_012541132.1
Coordinates 1778475..1778732 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M9O80_RS08490 (M9O80_08490) 1774085..1774660 + 576 WP_048271884.1 hypothetical protein -
M9O80_RS08495 (M9O80_08495) 1774837..1775673 + 837 WP_048271883.1 alpha/beta hydrolase -
M9O80_RS08500 (M9O80_08500) 1775880..1776851 + 972 WP_048271882.1 sensor domain-containing diguanylate cyclase -
M9O80_RS08505 (M9O80_08505) 1776848..1777948 - 1101 WP_048271881.1 AarF/UbiB family protein -
M9O80_RS08510 (M9O80_08510) 1778475..1778732 + 258 WP_012541132.1 antitoxin Antitoxin
M9O80_RS08515 (M9O80_08515) 1778733..1779065 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
M9O80_RS08525 (M9O80_08525) 1779388..1780824 + 1437 WP_016161429.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
M9O80_RS08535 (M9O80_08535) 1781197..1782651 - 1455 WP_064141536.1 AMP nucleosidase -
M9O80_RS08540 (M9O80_08540) 1782782..1783027 - 246 WP_008804171.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T246265 WP_008804165.1 NZ_CP097808:1778733-1779065 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT246265 WP_012541132.1 NZ_CP097808:1778475-1778732 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References