Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 285376..286007 Replicon chromosome
Accession NZ_CP097808
Organism Klebsiella variicola strain 17YN56

Toxin (Protein)


Gene name hicA Uniprot ID A0A1F2M5P8
Locus tag M9O80_RS01290 Protein ID WP_023323362.1
Coordinates 285376..285651 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID R4YIG2
Locus tag M9O80_RS01295 Protein ID WP_004181489.1
Coordinates 285648..286007 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M9O80_RS01270 (M9O80_01270) 280612..280947 + 336 WP_002921203.1 universal stress protein UspB -
M9O80_RS01275 (M9O80_01275) 281007..282503 - 1497 WP_008806938.1 inorganic phosphate transporter PitA -
M9O80_RS01280 (M9O80_01280) 282733..283926 + 1194 WP_012967106.1 NAD(P)/FAD-dependent oxidoreductase -
M9O80_RS01285 (M9O80_01285) 283966..284979 - 1014 WP_023323363.1 magnesium transporter -
M9O80_RS01290 (M9O80_01290) 285376..285651 + 276 WP_023323362.1 type II toxin-antitoxin system HicA family toxin Toxin
M9O80_RS01295 (M9O80_01295) 285648..286007 + 360 WP_004181489.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
M9O80_RS01300 (M9O80_01300) 286035..286436 - 402 WP_048268290.1 nickel-responsive transcriptional regulator NikR -
M9O80_RS01305 (M9O80_01305) 286424..287215 - 792 WP_012540373.1 nickel import ATP-binding protein NikE -
M9O80_RS01310 (M9O80_01310) 287212..287976 - 765 WP_008806946.1 nickel import ATP-binding protein NikD -
M9O80_RS01315 (M9O80_01315) 287976..288809 - 834 WP_088717475.1 nickel ABC transporter permease subunit NikC -
M9O80_RS01320 (M9O80_01320) 288806..289750 - 945 WP_032733899.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10260.86 Da        Isoelectric Point: 9.9665

>T246261 WP_023323362.1 NZ_CP097808:285376-285651 [Klebsiella variicola]
MEQQVLSLRNKQHHTLEQLFKIPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13313.02 Da        Isoelectric Point: 4.4605

>AT246261 WP_004181489.1 NZ_CP097808:285648-286007 [Klebsiella variicola]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCHEAGIEPYAHPEKMKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M5P8

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2M5Q1

References