Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4248043..4248674 Replicon chromosome
Accession NC_013941
Organism Escherichia coli O55:H7 str. CB9615

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag G2583_RS21650 Protein ID WP_001259385.1
Coordinates 4248399..4248674 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag G2583_RS21645 Protein ID WP_000593555.1
Coordinates 4248043..4248402 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G2583_RS21615 4243155..4243628 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
G2583_RS21620 4243625..4243906 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
G2583_RS21625 4243983..4245341 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
G2583_RS21630 4245334..4246842 + 1509 WP_013009296.1 carbohydrate kinase -
G2583_RS21635 4246832..4247101 + 270 WP_000933060.1 HPr family phosphocarrier protein -
G2583_RS21640 4247133..4247993 + 861 WP_001131788.1 class II aldolase -
G2583_RS21645 4248043..4248402 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
G2583_RS21650 4248399..4248674 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
G2583_RS21655 4248747..4249871 - 1125 WP_001301659.1 ABC transporter permease -
G2583_RS21660 4249871..4252606 - 2736 WP_000149105.1 ribosome-associated ATPase/putative transporter RbbA -
G2583_RS21665 4252603..4253670 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T24625 WP_001259385.1 NC_013941:c4248674-4248399 [Escherichia coli O55:H7 str. CB9615]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T24625 NC_013941:c4248674-4248399 [Escherichia coli O55:H7 str. CB9615]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT24625 WP_000593555.1 NC_013941:c4248402-4248043 [Escherichia coli O55:H7 str. CB9615]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT24625 NC_013941:c4248402-4248043 [Escherichia coli O55:H7 str. CB9615]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References