246234

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 385478..386115 Replicon chromosome
Accession NZ_CP097784
Organism Bacillus velezensis strain V 3.14

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag MF619_RS02175 Protein ID WP_003156187.1
Coordinates 385765..386115 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag MF619_RS02170 Protein ID WP_003156188.1
Coordinates 385478..385759 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MF619_RS02150 (MF619_000429) 381844..382443 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
MF619_RS02155 (MF619_000430) 382536..382901 + 366 WP_003156192.1 holo-ACP synthase -
MF619_RS02160 (MF619_000431) 383065..384072 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
MF619_RS02165 (MF619_000432) 384189..385358 + 1170 WP_025853726.1 alanine racemase -
MF619_RS02170 (MF619_000433) 385478..385759 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
MF619_RS02175 (MF619_000434) 385765..386115 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
MF619_RS02180 (MF619_000435) 386233..387054 + 822 WP_014304404.1 STAS domain-containing protein -
MF619_RS02185 (MF619_000436) 387059..387424 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
MF619_RS02190 (MF619_000437) 387427..387828 + 402 WP_003156178.1 anti-sigma regulatory factor -
MF619_RS02195 (MF619_000438) 387840..388847 + 1008 WP_025853725.1 PP2C family protein-serine/threonine phosphatase -
MF619_RS02200 (MF619_000439) 388911..389240 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
MF619_RS02205 (MF619_000440) 389237..389719 + 483 WP_003156173.1 anti-sigma B factor RsbW -
MF619_RS02210 (MF619_000441) 389685..390473 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
MF619_RS02215 (MF619_000442) 390473..391075 + 603 WP_003156170.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T246234 WP_003156187.1 NZ_CP097784:385765-386115 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT246234 WP_003156188.1 NZ_CP097784:385478-385759 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References