Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 315191..315828 Replicon chromosome
Accession NZ_CP097779
Organism Bacillus velezensis strain R 4.6

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag MF621_RS01530 Protein ID WP_003156187.1
Coordinates 315478..315828 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag MF621_RS01525 Protein ID WP_003156188.1
Coordinates 315191..315472 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MF621_RS01505 (MF621_000301) 311557..312156 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
MF621_RS01510 (MF621_000302) 312249..312614 + 366 WP_003156192.1 holo-ACP synthase -
MF621_RS01515 (MF621_000303) 312778..313785 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
MF621_RS01520 (MF621_000304) 313902..315071 + 1170 WP_025853726.1 alanine racemase -
MF621_RS01525 (MF621_000305) 315191..315472 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
MF621_RS01530 (MF621_000306) 315478..315828 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
MF621_RS01535 (MF621_000307) 315946..316767 + 822 WP_014304404.1 STAS domain-containing protein -
MF621_RS01540 (MF621_000308) 316772..317137 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
MF621_RS01545 (MF621_000309) 317140..317541 + 402 WP_003156178.1 anti-sigma regulatory factor -
MF621_RS01550 (MF621_000310) 317553..318560 + 1008 WP_025853725.1 PP2C family protein-serine/threonine phosphatase -
MF621_RS01555 (MF621_000311) 318624..318953 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
MF621_RS01560 (MF621_000312) 318950..319432 + 483 WP_003156173.1 anti-sigma B factor RsbW -
MF621_RS01565 (MF621_000313) 319398..320186 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
MF621_RS01570 (MF621_000314) 320186..320788 + 603 WP_003156170.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T246232 WP_003156187.1 NZ_CP097779:315478-315828 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT246232 WP_003156188.1 NZ_CP097779:315191-315472 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References