Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 143336..143979 Replicon plasmid pMS1050-1
Accession NZ_CP097727
Organism Escherichia coli strain MS1050

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag M9O78_RS24465 Protein ID WP_001044768.1
Coordinates 143336..143752 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag M9O78_RS24470 Protein ID WP_001261287.1
Coordinates 143749..143979 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M9O78_RS24445 (138366) 138366..139931 - 1566 WP_001128474.1 AAA family ATPase -
M9O78_RS24450 (140006) 140006..140422 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
M9O78_RS24455 (140419) 140419..140649 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
M9O78_RS24460 (141036) 141036..143174 + 2139 WP_001513523.1 AAA family ATPase -
M9O78_RS24465 (143336) 143336..143752 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
M9O78_RS24470 (143749) 143749..143979 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
M9O78_RS24475 (144398) 144398..145087 + 690 WP_001513524.1 RES family NAD+ phosphorylase -
M9O78_RS24480 (145119) 145119..145808 - 690 WP_001513525.1 helix-turn-helix domain-containing protein -
M9O78_RS24485 (146364) 146364..146582 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
M9O78_RS24490 (146584) 146584..146889 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
M9O78_RS24495 (146890) 146890..147672 + 783 WP_001513526.1 site-specific integrase -
M9O78_RS24500 (148372) 148372..148545 + 174 Protein_150 RepB family plasmid replication initiator protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA 1..203859 203859


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T246218 WP_001044768.1 NZ_CP097727:c143752-143336 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT246218 WP_001261287.1 NZ_CP097727:c143979-143749 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References