Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4485495..4485992 Replicon chromosome
Accession NZ_CP097726
Organism Escherichia coli strain MS1050

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag M9O78_RS21375 Protein ID WP_001173343.1
Coordinates 4485495..4485668 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag M9O78_RS21380 Protein ID WP_000398619.1
Coordinates 4485696..4485992 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M9O78_RS21365 (4482166) 4482166..4483623 + 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -
M9O78_RS21370 (4483860) 4483860..4485377 + 1518 WP_000003806.1 lysine--tRNA ligase -
M9O78_RS21375 (4485495) 4485495..4485668 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
M9O78_RS21380 (4485696) 4485696..4485992 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
M9O78_RS21385 (4486221) 4486221..4486493 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
M9O78_RS21390 (4486505) 4486505..4486735 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
M9O78_RS21395 (4486916) 4486916..4488547 + 1632 WP_001216477.1 sensor histidine kinase -
M9O78_RS21400 (4488544) 4488544..4489263 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T246212 WP_001173343.1 NZ_CP097726:c4485668-4485495 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT246212 WP_000398619.1 NZ_CP097726:c4485992-4485696 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References