Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2903622..2904406 Replicon chromosome
Accession NZ_CP097726
Organism Escherichia coli strain MS1050

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag M9O78_RS13855 Protein ID WP_000613626.1
Coordinates 2903912..2904406 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag M9O78_RS13850 Protein ID WP_001110447.1
Coordinates 2903622..2903915 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M9O78_RS13840 (2898772) 2898772..2899731 - 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
M9O78_RS13845 (2900304) 2900304..2903489 + 3186 WP_000827427.1 ribonuclease E -
M9O78_RS13850 (2903622) 2903622..2903915 + 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
M9O78_RS13855 (2903912) 2903912..2904406 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
M9O78_RS13860 (2904501) 2904501..2905454 - 954 WP_001212768.1 flagellar hook-associated protein FlgL -
M9O78_RS13865 (2905466) 2905466..2907109 - 1644 WP_000096524.1 flagellar hook-associated protein FlgK -
M9O78_RS13870 (2907175) 2907175..2908116 - 942 WP_001309406.1 flagellar assembly peptidoglycan hydrolase FlgJ -
M9O78_RS13875 (2908116) 2908116..2909213 - 1098 WP_000589320.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T246206 WP_000613626.1 NZ_CP097726:2903912-2904406 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT246206 WP_001110447.1 NZ_CP097726:2903622-2903915 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References