Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3602569..3603223 Replicon chromosome
Accession NC_013941
Organism Escherichia coli O55:H7 str. CB9615

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag G2583_RS18250 Protein ID WP_000244781.1
Coordinates 3602569..3602976 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag G2583_RS18255 Protein ID WP_000354046.1
Coordinates 3602957..3603223 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G2583_RS18230 3598526..3600259 - 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -
G2583_RS18235 3600265..3600975 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
G2583_RS18240 3601000..3601896 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
G2583_RS18245 3602008..3602529 + 522 WP_001055874.1 flavodoxin FldB -
G2583_RS18250 3602569..3602976 - 408 WP_000244781.1 protein YgfX Toxin
G2583_RS18255 3602957..3603223 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
G2583_RS18260 3603466..3604446 + 981 WP_000886055.1 tRNA-modifying protein YgfZ -
G2583_RS18265 3604523..3605182 - 660 WP_000250274.1 hemolysin III family protein -
G2583_RS18270 3605346..3605657 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
G2583_RS18275 3605702..3607135 + 1434 WP_001419180.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T24620 WP_000244781.1 NC_013941:c3602976-3602569 [Escherichia coli O55:H7 str. CB9615]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T24620 NC_013941:c3602976-3602569 [Escherichia coli O55:H7 str. CB9615]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT24620 WP_000354046.1 NC_013941:c3603223-3602957 [Escherichia coli O55:H7 str. CB9615]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT24620 NC_013941:c3603223-3602957 [Escherichia coli O55:H7 str. CB9615]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References