Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4236031..4236528 Replicon chromosome
Accession NZ_CP097721
Organism Escherichia coli strain MS1665

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag M9O79_RS20355 Protein ID WP_001173343.1
Coordinates 4236031..4236204 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag M9O79_RS20360 Protein ID WP_000398619.1
Coordinates 4236232..4236528 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M9O79_RS20345 (4232701) 4232701..4234158 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
M9O79_RS20350 (4234395) 4234395..4235912 + 1518 WP_001295063.1 lysine--tRNA ligase -
M9O79_RS20355 (4236031) 4236031..4236204 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
M9O79_RS20360 (4236232) 4236232..4236528 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
M9O79_RS20365 (4236755) 4236755..4237027 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
M9O79_RS20370 (4237039) 4237039..4237269 - 231 WP_000371699.1 4Fe-4S mono-cluster protein YjdI -
M9O79_RS20375 (4237450) 4237450..4239081 + 1632 WP_001216460.1 sensor histidine kinase -
M9O79_RS20380 (4239078) 4239078..4239797 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T246168 WP_001173343.1 NZ_CP097721:c4236204-4236031 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT246168 WP_000398619.1 NZ_CP097721:c4236528-4236232 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References