Detailed information of TA system TA246165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4039533..4041216
• Replicon: chromosome
• Accession: NZ_CP097721
• Organism: Escherichia coli strain MS1665

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: M9O79_RS19335
• Protein ID: WP_000361918.1
• Coordinates: 4040299..4041216 (+)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: I2UPX5
• Locus tag: M9O79_RS19330
• Protein ID: WP_000157137.1
• Coordinates: 4039533..4040306 (+)
• Length: 258 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M9O79_RS19315 (4035401)	4035401..4037485	+	2085	WP_001193073.1	protease Lon-related BREX system protein BrxL	-
M9O79_RS19320 (4037604)	4037604..4038953	+	1350	WP_000766444.1	phospholipase D-like domain-containing protein	-
M9O79_RS19325 (4039129)	4039129..4039449	-	321	Protein_3777	IS3 family transposase	-
M9O79_RS19330 (4039533)	4039533..4040306	+	774	WP_000157137.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
M9O79_RS19335 (4040299)	4040299..4041216	+	918	WP_000361918.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
M9O79_RS19340 (4041510)	4041510..4041677	+	168	WP_001356018.1	hypothetical protein	-
M9O79_RS19345 (4041730)	4041730..4042392	-	663	WP_000357928.1	hypothetical protein	-
M9O79_RS19350 (4042465)	4042465..4043106	-	642	Protein_3782	integrase arm-type DNA-binding domain-containing protein	-
M9O79_RS19355 (4043573)	4043573..4043716	-	144	Protein_3783	hypothetical protein	-
M9O79_RS19360 (4043801)	4043801..4043998	-	198	WP_001403937.1	DUF957 domain-containing protein	-
M9O79_RS19365 (4044018)	4044018..4044506	-	489	WP_000777665.1	DUF5983 family protein	-
M9O79_RS19370 (4044503)	4044503..4044880	-	378	WP_000854919.1	TA system toxin CbtA family protein	-
M9O79_RS19375 (4044927)	4044927..4045301	-	375	WP_001285608.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	-
M9O79_RS19380 (4045381)	4045381..4045602	-	222	WP_000692350.1	DUF987 domain-containing protein	-
M9O79_RS19385 (4045689)	4045689..4046165	-	477	WP_001186188.1	RadC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4039533..4053451	13918

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (13-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34962.10 Da
• Isoelectric Point: 4.9206

>T246165 WP_000361918.1 NZ_CP097721:4040299-4041216 [Escherichia coli]
MDKTSPYYRQVALLVRALPWVANESCFALKGGTAINLFIHDFPRLSVDIDLAWIPLESREVALPNVREALTRIAAELKAQ
TGIRAVLQTHNPDEMRIIVTTDDAQIKIEVSPVARGTLYPPQERDIVGAVEEEFGFAALPVVSLPDLYGGKLCAALDRQH
PRDFYDVKMLLDTQGIDRSIFNGFIVYLLSHNRPLSEVLNPRWKDITEPFQNEFSGMTFEEVSLEELKEVPERMLSTLKT
HFTQQDFDFLMSFKRGEPDWNLAPEPHIQNLPAVQWKLRNIGQMSEEKRAQSLEKLEKVMTEWLR

Nucleotide

• Length: 918 bp

Antitoxin

Protein

• Length: 258 a.a.
• Molecular weight: 29333.76 Da
• Isoelectric Point: 9.9889

>AT246165 WP_000157137.1 NZ_CP097721:4039533-4040306 [Escherichia coli]
MASKLNWLLQNTSPGSLVLQSWLTRNNISPSLAFKYAQNGWLIKLRAGVYARAGREPDWSDALWCLQNQLEAPVHLAGLS
SLSWQGRSHYLQLKQNQCWLSVENKAILPKWFKEFPAVEWLMVSALKLPDLDEKYRTELEVKGKRITASTPELAAYELLS
AVPQEISFEHAAELFQGLVNLNPRKVEKLLTLSQSVQTKRLYLFFASFYEHAWFKRIDTSKINLGSGNRQIVVNGKLNTQ
YQITVPENFTKKGISHG

Nucleotide

• Length: 774 bp

Structures

Antitoxin

• AlphaFold DB: A0A0H0N3W2