246163

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3448466..3449303 Replicon chromosome
Accession NZ_CP097721
Organism Escherichia coli strain MS1665

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag M9O79_RS16625 Protein ID WP_000227784.1
Coordinates 3448761..3449303 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag M9O79_RS16620 Protein ID WP_001297137.1
Coordinates 3448466..3448777 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M9O79_RS16595 (3443486) 3443486..3444433 + 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -
M9O79_RS16600 (3444455) 3444455..3446446 + 1992 WP_267320579.1 cytochrome o ubiquinol oxidase subunit I -
M9O79_RS16605 (3446436) 3446436..3447050 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
M9O79_RS16610 (3447050) 3447050..3447379 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
M9O79_RS16615 (3447391) 3447391..3448281 + 891 WP_000971336.1 heme o synthase -
M9O79_RS16620 (3448466) 3448466..3448777 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
M9O79_RS16625 (3448761) 3448761..3449303 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
M9O79_RS16630 (3449359) 3449359..3450294 - 936 WP_001297127.1 tetratricopeptide repeat protein -
M9O79_RS16635 (3450702) 3450702..3452066 + 1365 WP_001000978.1 MFS transporter -
M9O79_RS16640 (3452194) 3452194..3452685 - 492 WP_001138904.1 nucleotide binding protein YajQ -
M9O79_RS16645 (3452853) 3452853..3453764 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T246163 WP_000227784.1 NZ_CP097721:3448761-3449303 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT246163 WP_001297137.1 NZ_CP097721:3448466-3448777 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References