Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 3941086..3942806 Replicon chromosome
Accession NZ_CP097716
Organism Escherichia coli strain MS1718

Toxin (Protein)


Gene name pmenT Uniprot ID A0A7U9J2A7
Locus tag M9O77_RS18885 Protein ID WP_000312833.1
Coordinates 3941086..3941742 (+) Length 219 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID B7UP19
Locus tag M9O77_RS18890 Protein ID WP_000634203.1
Coordinates 3941739..3942806 (+) Length 356 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M9O77_RS18870 (3937001) 3937001..3937240 + 240 WP_024224214.1 hypothetical protein -
M9O77_RS18875 (3937280) 3937280..3939718 + 2439 WP_032180025.1 type I restriction-modification system subunit M -
M9O77_RS18880 (3939718) 3939718..3941073 + 1356 WP_224492247.1 restriction endonuclease subunit S -
M9O77_RS18885 (3941086) 3941086..3941742 + 657 WP_000312833.1 toxin-antitoxin system toxin DNA ADP-ribosyl transferase DarT Toxin
M9O77_RS18890 (3941739) 3941739..3942806 + 1068 WP_000634203.1 toxin-antitoxin system antitoxin DNA ADP-ribosyl glycohydrolase DarG Antitoxin
M9O77_RS18895 (3942825) 3942825..3945920 + 3096 WP_059337602.1 HsdR family type I site-specific deoxyribonuclease -
M9O77_RS18900 (3945920) 3945920..3946636 + 717 WP_001122107.1 SprT family zinc-dependent metalloprotease -
M9O77_RS18905 (3947490) 3947490..3947744 - 255 WP_001222075.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3932472..3947744 15272


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(17-217)

Antitoxin

Macro domain

(18-129)


Sequences

Toxin        


Download         Length: 219 a.a.        Molecular weight: 25233.56 Da        Isoelectric Point: 6.2145

>T246099 WP_000312833.1 NZ_CP097716:3941086-3941742 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Download         Length: 657 bp

Antitoxin


Download         Length: 356 a.a.        Molecular weight: 40672.70 Da        Isoelectric Point: 5.6927

>AT246099 WP_000634203.1 NZ_CP097716:3941739-3942806 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Download         Length: 1068 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9J2A7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9J2B9

References