Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 118697..119340 Replicon plasmid pMS1718-1
Accession NZ_CP097712
Organism Escherichia coli strain MS1737

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag M9O76_RS23890 Protein ID WP_001034044.1
Coordinates 118924..119340 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag M9O76_RS23885 Protein ID WP_001261286.1
Coordinates 118697..118927 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M9O76_RS23870 (113834) 113834..114064 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
M9O76_RS23875 (114061) 114061..114477 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
M9O76_RS23880 (114522) 114522..118316 - 3795 WP_001144732.1 hypothetical protein -
M9O76_RS23885 (118697) 118697..118927 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
M9O76_RS23890 (118924) 118924..119340 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
M9O76_RS23895 (119415) 119415..120980 + 1566 WP_001128474.1 AAA family ATPase -
M9O76_RS23900 (120965) 120965..121987 + 1023 WP_000361402.1 helicase UvrD -
M9O76_RS23910 (123294) 123294..124208 + 915 WP_000949004.1 iron/manganese ABC transporter substrate-binding protein SitA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD vat / iucA / iucB / iucC / iucD / iutA / iroB / iroC / iroD / iroE / iroN 1..188685 188685


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T246076 WP_001034044.1 NZ_CP097712:118924-119340 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT246076 WP_001261286.1 NZ_CP097712:118697-118927 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References