Detailed information of TA system TA024599

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 54779..55329
• Replicon: chromosome
• Accession: NC_013941
• Organism: Escherichia coli O55:H7 str. CB9615

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: G2583_RS00270
• Protein ID: WP_001160975.1
• Coordinates: 55015..55329 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: G2583_RS00265
• Protein ID: WP_000125566.1
• Coordinates: 54779..55012 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G2583_RS00240	49853..50140	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
G2583_RS00245	50198..51529	+	1332	WP_001183177.1	MFS transporter	-
G2583_RS00250	51637..52167	+	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
G2583_RS00255	52160..54022	+	1863	WP_000377186.1	glutathione-regulated potassium-efflux system protein KefC	-
G2583_RS00260	54214..54693	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
G2583_RS00265	54779..55012	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
G2583_RS00270	55015..55329	+	315	WP_001160975.1	CcdB family protein	Toxin
G2583_RS00275	55326..56174	-	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
G2583_RS00280	56181..56558	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
G2583_RS00285	56561..57382	-	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
G2583_RS00290	57379..58368	-	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
G2583_RS00295	58368..59654	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T24599 WP_001160975.1 NC_013941:55015-55329 [Escherichia coli O55:H7 str. CB9615]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T24599 NC_013941:55015-55329 [Escherichia coli O55:H7 str. CB9615]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT24599 WP_000125566.1 NC_013941:54779-55012 [Escherichia coli O55:H7 str. CB9615]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT24599 NC_013941:54779-55012 [Escherichia coli O55:H7 str. CB9615]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9