Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1902035..1902663 Replicon chromosome
Accession NC_013921
Organism Thermoanaerobacter italicus Ab9

Toxin (Protein)


Gene name mazF Uniprot ID B0KD57
Locus tag THIT_RS09260 Protein ID WP_003867472.1
Coordinates 1902035..1902385 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D3T4E5
Locus tag THIT_RS09265 Protein ID WP_012995808.1
Coordinates 1902388..1902663 (-) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
THIT_RS09240 1897528..1898262 - 735 WP_012995804.1 WecB/TagA/CpsF family glycosyltransferase -
THIT_RS09245 1898265..1899356 - 1092 WP_012995805.1 polysaccharide pyruvyl transferase CsaB -
THIT_RS09250 1899358..1901370 - 2013 WP_012995806.1 hypothetical protein -
THIT_RS09255 1901485..1901937 - 453 WP_012995807.1 hypothetical protein -
THIT_RS09260 1902035..1902385 - 351 WP_003867472.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
THIT_RS09265 1902388..1902663 - 276 WP_012995808.1 ribbon-helix-helix protein, CopG family Antitoxin
THIT_RS09270 1902761..1903918 - 1158 WP_012995809.1 alanine racemase -
THIT_RS09275 1903938..1904945 - 1008 WP_012995810.1 outer membrane lipoprotein carrier protein LolA -
THIT_RS09280 1904957..1906486 - 1530 WP_012995811.1 bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase -
THIT_RS09285 1906449..1906865 - 417 WP_012995812.1 holo-ACP synthase -
THIT_RS09290 1906960..1907325 - 366 WP_012995813.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-43)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12933.02 Da        Isoelectric Point: 6.4714

>T24594 WP_003867472.1 NC_013921:c1902385-1902035 [Thermoanaerobacter italicus Ab9]
MVIKRGDIFYADLSPVIGSEQGGIRPVLIIQNDIGNKYSPTVIVAAITSQINKAKLPTHVEINGAEYGLNKDSVVLLEQI
RTIDKKRLREKIGHFDQEMMEKVNEALQISLGLIDF

Download         Length: 351 bp

>T24594 NC_013921:c1902385-1902035 [Thermoanaerobacter italicus Ab9]
ATGGTGATAAAGAGGGGAGACATTTTTTATGCCGATTTAAGCCCTGTAATAGGCTCTGAACAGGGCGGTATTCGGCCTGT
ACTTATAATTCAAAATGATATTGGTAATAAATATAGTCCAACAGTAATAGTAGCGGCAATTACATCTCAGATCAACAAAG
CAAAATTGCCCACTCATGTTGAAATAAATGGGGCAGAATATGGACTTAACAAAGATTCTGTAGTATTGTTAGAGCAAATC
AGGACTATTGACAAAAAACGTTTAAGAGAAAAAATTGGCCATTTTGACCAGGAAATGATGGAAAAAGTAAATGAAGCTTT
GCAAATAAGTTTGGGGTTAATTGACTTTTAA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10503.30 Da        Isoelectric Point: 6.3661

>AT24594 WP_012995808.1 NC_013921:c1902663-1902388 [Thermoanaerobacter italicus Ab9]
VGETKRILVSLPQSLLEEVDVLAAMENRNRSEFIREAMKLYIREKKKIQIRESMKKGYLEMAAINSELAEMGLTAENECF
AGYEMKLKKCD

Download         Length: 276 bp

>AT24594 NC_013921:c1902663-1902388 [Thermoanaerobacter italicus Ab9]
GTGGGCGAAACAAAGCGAATACTTGTGAGCTTGCCGCAGAGTTTATTAGAAGAAGTCGATGTTCTTGCCGCTATGGAAAA
CAGGAATCGCAGTGAATTTATAAGAGAAGCGATGAAATTATATATACGGGAGAAGAAAAAAATTCAAATACGAGAGAGCA
TGAAAAAGGGATATCTTGAGATGGCAGCAATAAACAGTGAACTGGCGGAAATGGGCCTAACCGCAGAGAACGAATGTTTT
GCAGGATACGAAATGAAATTGAAAAAGTGTGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B3BRL3

Antitoxin

Source ID Structure
AlphaFold DB D3T4E5

References