Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 50096..50739 Replicon plasmid unnamed3
Accession NZ_CP097686
Organism Klebsiella pneumoniae strain IR12073_1

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag M3X91_RS27945 Protein ID WP_001044770.1
Coordinates 50096..50512 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag M3X91_RS27950 Protein ID WP_001261282.1
Coordinates 50509..50739 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M3X91_RS27925 (46199) 46199..46471 - 273 Protein_55 transposase -
M3X91_RS27935 (47453) 47453..48475 - 1023 WP_000361404.1 helicase UvrD -
M3X91_RS27940 (48460) 48460..50022 - 1563 WP_004206609.1 AAA family ATPase -
M3X91_RS27945 (50096) 50096..50512 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
M3X91_RS27950 (50509) 50509..50739 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
M3X91_RS27955 (50696) 50696..51157 + 462 WP_014343465.1 hypothetical protein -
M3X91_RS27960 (51318) 51318..52262 + 945 WP_011977810.1 hypothetical protein -
M3X91_RS27965 (52299) 52299..52691 + 393 WP_011977811.1 hypothetical protein -
M3X91_RS27970 (52749) 52749..53270 + 522 WP_094960442.1 hypothetical protein -
M3X91_RS27975 (53316) 53316..53519 + 204 WP_011977813.1 hypothetical protein -
M3X91_RS27980 (53549) 53549..54553 + 1005 WP_011977814.1 hypothetical protein -
M3X91_RS27985 (54737) 54737..55516 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / catA2 / blaCTX-M-65 - 1..153836 153836


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T245923 WP_001044770.1 NZ_CP097686:c50512-50096 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT245923 WP_001261282.1 NZ_CP097686:c50739-50509 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References