Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5303958..5304583 Replicon chromosome
Accession NZ_CP097658
Organism Klebsiella pneumoniae strain IR12099_1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag M3X93_RS26335 Protein ID WP_002882817.1
Coordinates 5303958..5304341 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M3X93_RS26340 Protein ID WP_004150355.1
Coordinates 5304341..5304583 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M3X93_RS26320 (M3X93_26320) 5301324..5302226 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
M3X93_RS26325 (M3X93_26325) 5302223..5302858 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
M3X93_RS26330 (M3X93_26330) 5302855..5303784 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
M3X93_RS26335 (M3X93_26335) 5303958..5304341 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
M3X93_RS26340 (M3X93_26340) 5304341..5304583 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M3X93_RS26345 (M3X93_26345) 5304788..5305705 + 918 WP_002882812.1 alpha/beta hydrolase -
M3X93_RS26350 (M3X93_26350) 5305719..5306660 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
M3X93_RS26355 (M3X93_26355) 5306705..5307142 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
M3X93_RS26360 (M3X93_26360) 5307139..5307999 - 861 WP_002882807.1 virulence factor BrkB family protein -
M3X93_RS26365 (M3X93_26365) 5307993..5308592 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T245777 WP_002882817.1 NZ_CP097658:c5304341-5303958 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT245777 WP_004150355.1 NZ_CP097658:c5304583-5304341 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References