Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5322347..5322972 Replicon chromosome
Accession NZ_CP097651
Organism Klebsiella pneumoniae strain NK01067

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag M8Y62_RS25530 Protein ID WP_012737091.1
Coordinates 5322347..5322730 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag M8Y62_RS25535 Protein ID WP_004150355.1
Coordinates 5322730..5322972 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8Y62_RS25515 (5319713) 5319713..5320615 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
M8Y62_RS25520 (5320612) 5320612..5321247 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
M8Y62_RS25525 (5321244) 5321244..5322173 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
M8Y62_RS25530 (5322347) 5322347..5322730 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
M8Y62_RS25535 (5322730) 5322730..5322972 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
M8Y62_RS25540 (5323177) 5323177..5324094 + 918 WP_012737090.1 alpha/beta hydrolase -
M8Y62_RS25545 (5324108) 5324108..5325049 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
M8Y62_RS25550 (5325094) 5325094..5325531 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
M8Y62_RS25555 (5325528) 5325528..5326388 - 861 WP_004146232.1 virulence factor BrkB family protein -
M8Y62_RS25560 (5326382) 5326382..5326981 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T245739 WP_012737091.1 NZ_CP097651:c5322730-5322347 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT245739 WP_004150355.1 NZ_CP097651:c5322972-5322730 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References