Detailed information of TA system TA024573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 3379721..3381177
• Replicon: chromosome
• Accession: NC_013861
• Organism: Legionella longbeachae NSW150

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: D3HLL7
• Locus tag: LLO_RS14685
• Protein ID: WP_012979426.1
• Coordinates: 3379721..3380572 (-)
• Length: 284 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: LLO_RS14690
• Protein ID: WP_012979427.1
• Coordinates: 3380569..3381177 (-)
• Length: 203 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLO_RS14660	3375331..3375684	-	354	WP_012979421.1	conjugal transfer transcriptional regulator TraJ	-
LLO_RS14665	3376078..3376422	+	345	WP_012979422.1	TraK family protein	-
LLO_RS14670	3376422..3377147	+	726	WP_012979423.1	conjugal transfer protein TraL	-
LLO_RS14675	3377147..3377584	+	438	WP_012979424.1	conjugal transfer protein TraM	-
LLO_RS14680	3377732..3379639	+	1908	WP_105223259.1	DUF2779 domain-containing protein	-
LLO_RS14685	3379721..3380572	-	852	WP_012979426.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LLO_RS14690	3380569..3381177	-	609	WP_012979427.1	AbiEi antitoxin N-terminal domain-containing protein	Antitoxin
LLO_RS14695	3381293..3383380	-	2088	WP_012979428.1	peptidase domain-containing ABC transporter	-
LLO_RS14700	3383377..3384387	-	1011	WP_012979429.1	HlyD family efflux transporter periplasmic adaptor subunit	-
LLO_RS14705	3384407..3385480	-	1074	WP_012979430.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (35-262)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (9-56)

Sequences

Toxin

Protein

• Length: 284 a.a.
• Molecular weight: 31793.67 Da
• Isoelectric Point: 6.7348

>T24573 WP_012979426.1 NC_013861:c3380572-3379721 [Legionella longbeachae NSW150]
MTKNTGESVRSRLKNIAIKEGSDFNAVLTRYGLERLLYRIGESEYSKQFLLKGALLFNLWYDMPHRPTKDIDLLGFGDND
LAYVKQTFSKICRISTDDGISFLSESVTVDTIKKDGGYTGARVELFGELAKAKIKIQIDIGYGDAVTPGPIDAHYPVLLS
DLPAPKIWTYPIYTVIAEKLHAIALLGMANSRLKDYLDLYVLLSNEQIDNQVLAKAIQATFNRRGMDLPEALPVGLTDEF
ANDPSRESMWKAFLRKNELEQKPLTEVIAVIRNLIQVPYSLAK

Nucleotide

• Length: 852 bp

>T24573 NC_013861:c3380572-3379721 [Legionella longbeachae NSW150]
ATGACAAAAAATACTGGTGAGTCTGTACGCTCACGTTTGAAGAATATAGCTATTAAAGAAGGTTCTGATTTTAATGCGGT
ATTGACTCGTTATGGTCTAGAGCGTTTGTTATATCGAATTGGAGAATCAGAATATTCTAAACAGTTTTTGTTGAAAGGTG
CCTTATTATTTAACTTATGGTATGACATGCCTCATCGCCCAACTAAAGATATTGATTTATTGGGGTTTGGTGATAATGAT
CTAGCGTATGTTAAACAAACGTTCAGTAAAATTTGCAGAATCTCTACAGATGATGGAATAAGTTTCCTGTCTGAATCAGT
GACGGTTGACACTATCAAAAAAGATGGGGGTTATACTGGAGCAAGAGTTGAGTTATTTGGGGAATTGGCGAAAGCAAAGA
TTAAAATACAAATTGATATAGGTTATGGTGATGCAGTTACCCCTGGGCCAATAGATGCCCATTATCCCGTGCTTCTTAGT
GATCTTCCTGCCCCTAAAATCTGGACTTATCCAATTTATACGGTAATTGCAGAAAAGCTGCACGCAATTGCATTATTGGG
CATGGCGAATAGTCGATTAAAAGACTACTTGGATCTTTATGTATTATTAAGTAATGAACAGATAGATAACCAAGTCTTGG
CTAAAGCTATACAGGCTACATTTAATCGTCGAGGTATGGATCTTCCTGAAGCGCTGCCTGTTGGCCTGACCGATGAGTTT
GCTAACGATCCAAGCAGGGAATCAATGTGGAAAGCCTTTTTGAGAAAAAATGAACTAGAGCAGAAACCATTAACTGAAGT
TATTGCTGTGATCAGAAATTTGATTCAAGTGCCATATTCTTTGGCAAAGTAA

Antitoxin

Protein

• Length: 203 a.a.
• Molecular weight: 23109.22 Da
• Isoelectric Point: 9.9450

>AT24573 WP_012979427.1 NC_013861:c3381177-3380569 [Legionella longbeachae NSW150]
MKKEDNYKQKVLQMASKTGVIRMSDLTKKGITRATFSRLVREKKLEKLAPGLYCLPQAELSEKESLVIIASRVPQAVFCL
LTALQIHDLTTQLPRKVWIAMPKGSHAPKMDYPPLKMVQYSDEAFSEGIEIIESDNIKLRVYNRAKTIADCFKHRNKIGL
DVAIEALKEAYTKNKVTVDELWHYAKICRVANVMRPYIEAIQ

Nucleotide

• Length: 609 bp

>AT24573 NC_013861:c3381177-3380569 [Legionella longbeachae NSW150]
ATGAAGAAAGAAGACAACTACAAACAAAAAGTACTCCAGATGGCTAGCAAAACGGGCGTTATTCGAATGTCCGATCTGAC
CAAGAAGGGGATTACTAGAGCGACCTTTTCAAGGCTTGTTAGAGAAAAAAAGCTTGAAAAACTCGCTCCTGGGCTTTATT
GCTTGCCCCAAGCTGAGCTTTCAGAAAAAGAAAGCCTGGTAATTATAGCAAGCCGAGTTCCGCAAGCGGTGTTTTGTCTG
CTAACTGCTTTGCAAATCCATGATCTAACAACACAGCTACCTCGAAAAGTATGGATAGCTATGCCCAAAGGCAGTCATGC
GCCAAAAATGGATTATCCGCCGCTAAAAATGGTGCAATATTCAGATGAAGCCTTTAGCGAAGGAATTGAAATTATAGAAT
CAGATAATATTAAACTAAGGGTGTATAACCGTGCCAAGACAATTGCAGATTGTTTCAAACATCGCAATAAAATTGGACTT
GATGTGGCCATTGAAGCGCTCAAAGAGGCTTATACAAAAAATAAAGTCACAGTTGATGAACTATGGCATTATGCGAAAAT
TTGTCGCGTTGCAAACGTAATGCGTCCTTATATTGAGGCAATTCAATGA

Structures

Toxin

• AlphaFold DB: D3HLL7