Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 110685..111421 Replicon plasmid pKqu-1
Accession NZ_CP097647
Organism Klebsiella quasipneumoniae strain NE1

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag M8232_RS25415 Protein ID WP_003026803.1
Coordinates 110685..111167 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag M8232_RS25420 Protein ID WP_003026799.1
Coordinates 111155..111421 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8232_RS25400 (M8232_25400) 107664..108236 + 573 WP_250202730.1 recombinase family protein -
M8232_RS25405 (M8232_25405) 108245..109063 + 819 WP_250202731.1 abortive infection family protein -
M8232_RS25410 (M8232_25410) 109134..110477 - 1344 WP_136697913.1 ISNCY family transposase -
M8232_RS25415 (M8232_25415) 110685..111167 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
M8232_RS25420 (M8232_25420) 111155..111421 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
M8232_RS25425 (M8232_25425) 111682..112650 + 969 WP_072202995.1 IS5 family transposase -
M8232_RS25430 (M8232_25430) 112754..113874 + 1121 WP_085950818.1 IS3-like element ISSen4 family transposase -
M8232_RS25435 (M8232_25435) 114102..115055 + 954 WP_001198018.1 cation diffusion facilitator family transporter -
M8232_RS25440 (M8232_25440) 115167..115541 + 375 WP_011977761.1 cytochrome b562 -
M8232_RS25445 (M8232_25445) 115979..116077 + 99 Protein_133 cytochrome b/b6 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkA / mrkB / mrkC / mrkF / mrkJ / pla 1..181209 181209
- inside IScluster/Tn - - 104911..117128 12217


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T245721 WP_003026803.1 NZ_CP097647:c111167-110685 [Klebsiella quasipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT245721 WP_003026799.1 NZ_CP097647:c111421-111155 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References