Detailed information of TA system TA024572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 28952..29484
• Replicon: plasmid pAB510f
• Accession: NC_013860
• Organism: Azospirillum sp. B510

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D3P806
• Locus tag: AZL_RS32380
• Protein ID: WP_012978574.1
• Coordinates: 29161..29484 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AZL_RS32375
• Protein ID: WP_042446943.1
• Coordinates: 28952..29164 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZL_RS32345	24004..25932	-	1929	WP_148219819.1	DUF839 domain-containing protein	-
AZL_RS32350	26102..26374	-	273	WP_012978568.1	hypothetical protein	-
AZL_RS32355	26385..26993	-	609	WP_012978569.1	NUDIX hydrolase	-
AZL_RS32360	27022..27405	-	384	WP_012978570.1	type II toxin-antitoxin system VapC family toxin	-
AZL_RS32365	27413..27646	-	234	WP_012978571.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
AZL_RS32370	27758..28753	-	996	WP_042446941.1	hypothetical protein	-
AZL_RS32375	28952..29164	+	213	WP_042446943.1	antitoxin MazE family protein	Antitoxin
AZL_RS32380	29161..29484	+	324	WP_012978574.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AZL_RS32385	29526..30674	+	1149	WP_012978575.1	glycoside hydrolase	-
AZL_RS32390	30741..31859	+	1119	WP_012978576.1	Rieske 2Fe-2S domain-containing protein	-
AZL_RS32395	31965..33599	+	1635	WP_012978577.1	DUF1800 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd	1..261596	261596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-101)

Antitoxin

• Antitoxin MazE-like (8-69)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11550.41 Da
• Isoelectric Point: 8.8545

>T24572 WP_012978574.1 NC_013860:29161-29484 [Azospirillum sp. B510]
VRRGDLVTVAMQGDSGKPRPALVIQSDLFDGHPSVTILPITGELVEAPLFRLSVDPTPGNGLRKRSQVMVDKVQTIARDK
IGEAFGRLGDDSMLAVNRAFLLWIGLA

Nucleotide

• Length: 324 bp

>T24572 NC_013860:29161-29484 [Azospirillum sp. B510]
GTGAGGCGCGGCGATCTGGTGACGGTCGCCATGCAAGGCGATTCCGGGAAACCGCGTCCGGCGCTGGTCATCCAGTCCGA
TCTGTTCGACGGGCATCCCAGCGTCACCATCCTGCCGATCACGGGCGAGCTGGTGGAGGCGCCGTTGTTCCGCCTGTCGG
TCGACCCGACGCCAGGCAACGGCCTGCGCAAACGCTCGCAGGTGATGGTCGACAAGGTCCAGACGATCGCCCGCGACAAG
ATCGGCGAGGCGTTCGGACGGTTGGGGGATGACAGCATGCTCGCGGTGAACCGGGCGTTCCTCCTCTGGATCGGACTGGC
CTGA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 8417.50 Da
• Isoelectric Point: 11.3867

>AT24572 WP_042446943.1 NC_013860:28952-29164 [Azospirillum sp. B510]
MSTPLSFRVQRHRDNLRAAGLRPLQIWVPDTRRPGFATECRRQSRLVRDTLREQETLDWLDAVRDREGWE

Nucleotide

• Length: 213 bp

>AT24572 NC_013860:28952-29164 [Azospirillum sp. B510]
ATGTCCACACCGCTTTCCTTCCGGGTCCAGCGCCACCGCGACAACCTGCGTGCCGCCGGGCTGCGTCCCTTGCAGATCTG
GGTGCCGGACACCCGCCGGCCCGGCTTCGCCACCGAATGCCGGCGCCAGTCGCGGCTGGTCCGGGACACCTTGCGGGAAC
AGGAGACGCTGGACTGGCTGGACGCGGTCCGCGATAGGGAGGGCTGGGAGTGA

Structures

Toxin

• AlphaFold DB: D3P806