Detailed information of TA system TA245715

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2491575..2493163
• Replicon: chromosome
• Accession: NZ_CP097646
• Organism: Klebsiella quasipneumoniae strain NE1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: M8232_RS12065
• Protein ID: WP_004205919.1
• Coordinates: 2491575..2492897 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2A5MFC9
• Locus tag: M8232_RS12070
• Protein ID: WP_004205921.1
• Coordinates: 2492897..2493163 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M8232_RS12030 (2486657)	2486657..2486875	+	219	WP_002904808.1	ParD-like family protein	-
M8232_RS12035 (2486856)	2486856..2487638	+	783	WP_048294851.1	type I methionyl aminopeptidase	-
M8232_RS12040 (2487907)	2487907..2489022	+	1116	WP_156234679.1	serine hydrolase domain-containing protein	-
M8232_RS12045 (2489009)	2489009..2489353	+	345	WP_004205915.1	hypothetical protein	-
M8232_RS12050 (2489382)	2489382..2489915	+	534	WP_004205916.1	hypothetical protein	-
M8232_RS12055 (2489918)	2489918..2490124	+	207	WP_004205917.1	helix-turn-helix transcriptional regulator	-
M8232_RS12060 (2490266)	2490266..2491558	+	1293	WP_004205918.1	glycoside hydrolase family 10 protein	-
M8232_RS12065 (2491575)	2491575..2492897	-	1323	WP_004205919.1	type II toxin-antitoxin system HipA family toxin	Toxin
M8232_RS12070 (2492897)	2492897..2493163	-	267	WP_004205921.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
M8232_RS12075 (2493446)	2493446..2493772	-	327	WP_069602708.1	hydroxyisourate hydrolase	-
M8232_RS12080 (2493769)	2493769..2494269	-	501	WP_017900378.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
M8232_RS12085 (2494247)	2494247..2495626	-	1380	WP_156234680.1	nucleobase:cation symporter-2 family protein	-
M8232_RS12090 (2495972)	2495972..2497126	+	1155	WP_004205928.1	FAD-dependent urate hydroxylase HpxO	-
M8232_RS12095 (2497107)	2497107..2498030	-	924	WP_004205929.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	blaOKP-B-15	mgtC / iroE / sciN/tssJ / tssG / tssF / icmF/tssM / KPHS_23120 / tle1 / tli1 / tli1 / tli1 / tli1 / tle1 / tli1 / tli1 / tli1 / vgrG/tssI / clpV/tssH / hcp/tssD / dotU/tssL / vasE/tssK / vipB/tssC / vipA/tssB	2330318..2871387	541069

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49013.27 Da
• Isoelectric Point: 8.2655

>T245715 WP_004205919.1 NZ_CP097646:c2492897-2491575 [Klebsiella quasipneumoniae]
MATLTTWMNNARVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRERIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIINTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILGEFADRVP
QAIESARQTLPSDFSAHVWRAITENMLKLHARLQQGLQAG

Nucleotide

• Length: 1323 bp

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9940.38 Da
• Isoelectric Point: 7.1997

>AT245715 WP_004205921.1 NZ_CP097646:c2493163-2492897 [Klebsiella quasipneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETAMIP
DNTTDLDW

Nucleotide

• Length: 267 bp

Structures

Antitoxin

• AlphaFold DB: A0A2A5MFC9