Detailed information of TA system TA024571

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2874792..2875785
• Replicon: chromosome
• Accession: NC_013854
• Organism: Azospirillum sp. B510

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: AZL_RS13435
• Protein ID: WP_012975085.1
• Coordinates: 2875246..2875785 (+)
• Length: 180 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: D3NXN1
• Locus tag: AZL_RS13430
• Protein ID: WP_012975084.1
• Coordinates: 2874792..2875241 (+)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZL_RS13405	2870821..2871348	-	528	WP_052293691.1	demethoxyubiquinone hydroxylase family protein	-
AZL_RS13410	2871402..2871935	-	534	WP_052293692.1	disulfide bond formation protein B	-
AZL_RS13420	2872263..2872616	-	354	WP_012975082.1	carboxymuconolactone decarboxylase family protein	-
AZL_RS13425	2873435..2874685	+	1251	WP_042443826.1	site-specific integrase	-
AZL_RS13430	2874792..2875241	+	450	WP_012975084.1	excisionase family DNA-binding protein	Antitoxin
AZL_RS13435	2875246..2875785	+	540	WP_012975085.1	PIN domain-containing protein	Toxin
AZL_RS13440	2875879..2877393	+	1515	WP_012972683.1	IS21-like element ISAzs2 family transposase	-
AZL_RS13445	2877404..2878159	+	756	WP_012972684.1	IS21-like element ISAzs2 family helper ATPase IstB	-
AZL_RS35500	2879683..2880036	+	354	WP_158305976.1	hypothetical protein	-
AZL_RS36595	2880565..2880738	+	174	WP_173380482.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2871402..2922810	51408

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (79-124)

Sequences

Toxin

Protein

• Length: 180 a.a.
• Molecular weight: 20348.17 Da
• Isoelectric Point: 6.0724

>T24571 WP_012975085.1 NC_013854:2875246-2875785 [Azospirillum sp. B510]
VSRFTVVFDACCLYPAPLRDFLMELTTAELFRAKWTWAIHEEWTRNLLHNRPDLTREAVKRTCALMNRAAPDCLVEGYEH
LIPVVELPDADDRHVLAAAIHARADAIVTFNLKDFPAAELGKFNVEAIHPDDFISYQFDLHEPAVVIAAQRCLHISAEST
QRERRKAPGQNGESAQPFR

Nucleotide

• Length: 540 bp

>T24571 NC_013854:2875246-2875785 [Azospirillum sp. B510]
GTGTCGCGCTTCACCGTTGTCTTCGATGCCTGTTGCCTCTACCCCGCCCCACTGCGGGATTTCCTCATGGAGTTGACGAC
GGCGGAGCTGTTCCGGGCGAAATGGACATGGGCGATCCATGAGGAATGGACGCGTAACCTGCTCCACAACCGCCCGGACC
TGACGCGGGAGGCCGTGAAACGCACCTGTGCCTTGATGAACCGTGCCGCCCCGGATTGCCTCGTGGAAGGGTATGAGCAC
CTGATCCCCGTGGTCGAACTGCCGGACGCCGATGACCGGCACGTCCTGGCAGCCGCGATCCACGCCCGCGCCGATGCCAT
CGTGACCTTCAACCTGAAGGACTTTCCGGCCGCGGAACTGGGTAAGTTCAACGTCGAGGCCATCCACCCGGACGACTTCA
TCAGCTACCAGTTCGACCTGCACGAACCGGCCGTTGTGATCGCCGCGCAGAGGTGCCTGCATATTTCGGCGGAAAGCACC
CAGCGGGAACGGCGGAAAGCGCCCGGGCAGAACGGTGAAAGCGCCCAGCCGTTTCGGTGA

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 16546.83 Da
• Isoelectric Point: 6.3819

>AT24571 WP_012975084.1 NC_013854:2874792-2875241 [Azospirillum sp. B510]
MNALLEKPDTVVPSEEDAALAAESSRILASRPSEGEFRVHLDDGQTLALPRAVKQLLAHLLTEMSHGNAVTLIPIHAEMT
TQEAADYLNVSRPHLVKLLEEGTIPFHKVGSHRRVRFRDLKSYKDRITADRAKALDELAAQAQELNMGY

Nucleotide

• Length: 450 bp

>AT24571 NC_013854:2874792-2875241 [Azospirillum sp. B510]
ATGAACGCCCTACTCGAAAAGCCGGACACCGTCGTTCCTTCCGAGGAAGACGCCGCGCTGGCGGCCGAGTCGAGCCGTAT
CCTGGCCTCCAGGCCTTCCGAAGGAGAGTTCCGGGTTCACCTGGACGACGGTCAGACGCTGGCCTTGCCGCGCGCTGTGA
AGCAACTCCTTGCCCATCTGCTGACGGAGATGTCGCATGGCAACGCCGTCACGCTGATCCCGATCCATGCTGAGATGACC
ACCCAGGAAGCGGCCGACTATCTGAACGTCAGCCGGCCCCATTTGGTGAAGCTGTTGGAAGAGGGAACAATCCCCTTCCA
CAAGGTCGGTTCCCACCGCCGGGTGCGCTTCCGTGATCTGAAGAGCTATAAAGACCGGATCACGGCTGATCGCGCCAAGG
CTCTGGACGAGTTGGCGGCCCAGGCCCAGGAACTCAACATGGGGTACTGA

Structures

Antitoxin

• AlphaFold DB: D3NXN1