Detailed information of TA system TA024570

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): zeta-pezA/zeta-couple_hipB
• Location: 1089095..1090341
• Replicon: chromosome
• Accession: NC_013853
• Organism: Streptococcus mitis B6

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: D3H903
• Locus tag: SMI_RS05580
• Protein ID: WP_000405352.1
• Coordinates: 1089095..1089865 (-)
• Length: 257 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: B6ZCC4
• Locus tag: SMI_RS05585
• Protein ID: WP_000579612.1
• Coordinates: 1089865..1090341 (-)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMI_RS05550	1084686..1085252	-	567	WP_000180154.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-
SMI_RS05555	1085345..1085569	-	225	WP_000864436.1	hypothetical protein	-
SMI_RS05560	1085660..1086274	-	615	Protein_1048	hypothetical protein	-
SMI_RS05565	1086597..1087319	-	723	WP_164925559.1	hypothetical protein	-
SMI_RS05570	1087350..1087595	-	246	Protein_1050	hypothetical protein	-
SMI_RS05575	1087846..1088640	-	795	WP_164925560.1	hypothetical protein	-
SMI_RS05580	1089095..1089865	-	771	WP_000405352.1	zeta toxin family protein	Toxin
SMI_RS05585	1089865..1090341	-	477	WP_000579612.1	helix-turn-helix domain-containing protein	Antitoxin
SMI_RS05590	1090617..1091072	-	456	WP_025173400.1	hypothetical protein	-
SMI_RS05595	1091340..1093085	-	1746	WP_000423809.1	glycoside hydrolase family 13 protein	-
SMI_RS05600	1093196..1093504	-	309	Protein_1056	phosphoribosylanthranilate isomerase	-
SMI_RS05605	1093568..1094449	-	882	WP_000710090.1	diacylglycerol kinase family lipid kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1077398..1106453	29055

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 257 a.a.
• Molecular weight: 29329.21 Da
• Isoelectric Point: 6.9752

>T24570 WP_000405352.1 NC_013853:c1089865-1089095 [Streptococcus mitis B6]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTELSHLGYNLLIEGTLRTIDVPKKTAQLLKSKGYEVQLALIATKPELSYLSTLIRYEE
LYSINPTQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTTSAATVLHDLLFGEWNQVEKEML
RSGEERLKDLTNRNGC

Nucleotide

• Length: 771 bp

>T24570 NC_013853:c1089865-1089095 [Streptococcus mitis B6]
ATGGAAATCCAAGATTATACTGATAGTGAATTCAAACATGCTTTAGCACGGAATCTTCGTTCTCTGACAAGAGGAAAAAA
GTCCAGTAAGCAACCTATAGCGATTTTGCTTGGAGGGCAAAGTGGTGCTGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGCGATAGTTTTCGCTCTCAACATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGATAGTGTAGAATACACCAAAGATTTTGCAGGTAAAATGGTAGAGTCTTTAGTAACAGAATTGAGTCA
TTTGGGATACAATCTTTTGATAGAGGGAACTTTACGAACTATTGATGTTCCAAAGAAAACAGCACAACTCTTGAAAAGTA
AGGGATATGAAGTACAATTAGCCTTAATTGCAACAAAGCCCGAATTGTCCTATCTGAGCACTCTTATCCGATACGAAGAA
CTGTACTCTATCAATCCAACTCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
TACACGGCAATTGGAAGAACTAGCTATCTTTGAAAGAATTCAAATTTACCAACGAGATAGAAGTTGTGTATATGATTCAA
AAGAAAATACAACTTCAGCAGCAACCGTTCTACATGATTTACTATTTGGAGAATGGAATCAAGTGGAGAAAGAGATGCTT
AGATCTGGAGAGGAAAGATTGAAAGATTTAACTAATCGAAATGGTTGTTAG

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18245.68 Da
• Isoelectric Point: 4.4188

>AT24570 WP_000579612.1 NC_013853:c1090341-1089865 [Streptococcus mitis B6]
MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT24570 NC_013853:c1090341-1089865 [Streptococcus mitis B6]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACACAACCCGAATTTGCACGAATTATAGGAATTTC
TCGAAATAGCTTGAGTCGTTATGAAAATGGAACTAGTTCAGTCTCTACGGAACTAATAGATATCATCTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTAAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTTTTATCTCGACTCTATCGCTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATTTATCTGACTTGATTCATACGAATATCTATTTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGCGGTTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAGCGGATGGTAGCTTAA

Structures

Toxin

• AlphaFold DB: D3H903

Antitoxin

• AlphaFold DB: B6ZCC4