Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 149293..149936 Replicon plasmid pKPC2_140253
Accession NZ_CP097627
Organism Klebsiella pneumoniae strain 140253

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag M9P58_RS27925 Protein ID WP_001044770.1
Coordinates 149293..149709 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag M9P58_RS27930 Protein ID WP_001261282.1
Coordinates 149706..149936 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M9P58_RS27905 (145396) 145396..145668 - 273 Protein_189 transposase -
M9P58_RS27915 (146650) 146650..147672 - 1023 WP_000361404.1 helicase UvrD -
M9P58_RS27920 (147657) 147657..149219 - 1563 WP_004206609.1 AAA family ATPase -
M9P58_RS27925 (149293) 149293..149709 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
M9P58_RS27930 (149706) 149706..149936 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
M9P58_RS27935 (149893) 149893..150354 + 462 WP_014343465.1 hypothetical protein -
M9P58_RS27940 (150515) 150515..151459 + 945 WP_011977810.1 hypothetical protein -
M9P58_RS27945 (151496) 151496..151888 + 393 WP_011977811.1 hypothetical protein -
M9P58_RS27950 (151946) 151946..152467 + 522 WP_013214008.1 hypothetical protein -
M9P58_RS27955 (152513) 152513..152716 + 204 WP_011977813.1 hypothetical protein -
M9P58_RS27960 (152746) 152746..153750 + 1005 WP_011977814.1 hypothetical protein -
M9P58_RS27965 (153934) 153934..154713 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid rmtB / blaTEM-1B / blaCTX-M-65 / blaKPC-2 - 1..155793 155793


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T245695 WP_001044770.1 NZ_CP097627:c149709-149293 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT245695 WP_001261282.1 NZ_CP097627:c149936-149706 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References