Detailed information of TA system TA024567

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelB(antitoxin)
• Location: 106507..107131
• Replicon: chromosome
• Accession: NC_013853
• Organism: Streptococcus mitis B6

Toxin (Protein)

• Gene name: parE
• Uniprot ID: D3H6B0
• Locus tag: SMI_RS00520
• Protein ID: WP_000395513.1
• Coordinates: 106784..107131 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: D3H6A9
• Locus tag: SMI_RS00515
• Protein ID: WP_001101476.1
• Coordinates: 106507..106794 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMI_RS00485	102068..103120	+	1053	WP_000769776.1	HAMP domain-containing histidine kinase	-
SMI_RS00490	103315..103926	+	612	WP_000092756.1	30S ribosomal protein S4	-
SMI_RS00495	104022..104240	-	219	Protein_92	tyrosine-type recombinase/integrase	-
SMI_RS00500	104903..105118	-	216	WP_000420651.1	hypothetical protein	-
SMI_RS00505	105149..105358	-	210	WP_001044017.1	hypothetical protein	-
SMI_RS00510	105390..105758	-	369	WP_000376152.1	hypothetical protein	-
SMI_RS00515	106507..106794	+	288	WP_001101476.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
SMI_RS00520	106784..107131	+	348	WP_000395513.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SMI_RS00525	107642..108448	+	807	WP_000834989.1	hypothetical protein	-
SMI_RS00530	108470..109717	+	1248	WP_000134331.1	toxic anion resistance protein	-
SMI_RS00535	110229..110567	-	339	WP_000711088.1	Cd(II)/Zn(II)-sensing metalloregulatory transcriptional regulator CadX	-
SMI_RS00540	110579..111205	-	627	WP_164925503.1	CadD family cadmium resistance transporter	-
SMI_RS00550	111457..111606	-	150	WP_001265622.1	50S ribosomal protein L33	-
SMI_RS00555	111622..111804	-	183	WP_000290417.1	50S ribosomal protein L32	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	112037..113203	1166

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (10-108)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (7-70)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13361.30 Da
• Isoelectric Point: 5.1972

>T24567 WP_000395513.1 NC_013853:106784-107131 [Streptococcus mitis B6]
MEFNENAYSLIISETVQEQLRGIKDYISANYFSEQAGANTVRNILYGLERLEVFPEAGFDADERVGCIIYPPLKTRCIIL
GDYLAFYHILEDRKAVFVSDIIHSKQDYIRLFKKK

Nucleotide

• Length: 348 bp

>T24567 NC_013853:106784-107131 [Streptococcus mitis B6]
TTGGAATTTAACGAGAACGCATATAGCCTCATTATCTCTGAAACGGTACAAGAACAGCTAAGAGGTATCAAAGACTATAT
CTCAGCTAACTACTTTTCAGAACAGGCAGGAGCAAACACAGTTAGGAATATTCTTTATGGTTTGGAGCGTCTTGAAGTCT
TCCCAGAAGCAGGATTTGATGCTGACGAAAGAGTAGGATGTATTATATATCCACCCCTCAAAACTCGATGTATCATTTTA
GGAGATTATCTAGCCTTCTATCATATTTTAGAGGACAGAAAAGCTGTCTTTGTATCAGATATTATTCATAGCAAACAGGA
CTACATCCGCTTGTTCAAGAAAAAGTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10922.34 Da
• Isoelectric Point: 4.7119

>AT24567 WP_001101476.1 NC_013853:106507-106794 [Streptococcus mitis B6]
MNVLEKNTQVNFKTNRDLLEKAKAIITAQNLDMTASFNLFLEHIVEHKSLPFETQVDKEREELLSGLRAEIARSFDDLEQ
GRTYSLDEVRANLGI

Nucleotide

• Length: 288 bp

>AT24567 NC_013853:106507-106794 [Streptococcus mitis B6]
ATGAATGTTTTAGAAAAAAATACACAGGTAAATTTTAAGACTAACAGAGACTTGTTAGAGAAGGCTAAAGCTATTATCAC
AGCGCAAAATCTTGATATGACAGCCAGCTTTAACTTGTTTTTGGAACACATTGTAGAACATAAATCCTTGCCATTTGAAA
CTCAAGTAGATAAAGAAAGAGAAGAACTTCTATCAGGGTTGCGTGCTGAAATTGCCCGTAGCTTTGACGATTTAGAACAG
GGAAGAACTTACAGCCTTGATGAAGTGAGGGCTAACCTTGGAATTTAA

Structures

Toxin

• AlphaFold DB: D3H6B0

Antitoxin

• AlphaFold DB: D3H6A9