Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1329710..1330347 Replicon chromosome
Accession NZ_CP097594
Organism Bacillus velezensis strain UA0182

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag M8968_RS06650 Protein ID WP_003156187.1
Coordinates 1329997..1330347 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag M8968_RS06645 Protein ID WP_003156188.1
Coordinates 1329710..1329991 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M8968_RS06625 1326075..1326674 - 600 WP_032872997.1 rhomboid family intramembrane serine protease -
M8968_RS06630 1326767..1327132 + 366 WP_007609580.1 holo-ACP synthase -
M8968_RS06635 1327297..1328304 + 1008 WP_032872999.1 outer membrane lipoprotein carrier protein LolA -
M8968_RS06640 1328421..1329590 + 1170 WP_032873001.1 alanine racemase -
M8968_RS06645 1329710..1329991 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
M8968_RS06650 1329997..1330347 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
M8968_RS06655 1330465..1331286 + 822 WP_003156182.1 STAS domain-containing protein -
M8968_RS06660 1331291..1331656 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
M8968_RS06665 1331659..1332060 + 402 WP_003156178.1 anti-sigma regulatory factor -
M8968_RS06670 1332072..1333079 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
M8968_RS06675 1333143..1333472 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
M8968_RS06680 1333469..1333951 + 483 WP_007609591.1 anti-sigma B factor RsbW -
M8968_RS06685 1333917..1334705 + 789 WP_032873003.1 RNA polymerase sigma factor SigB -
M8968_RS06690 1334705..1335307 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T245649 WP_003156187.1 NZ_CP097594:1329997-1330347 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT245649 WP_003156188.1 NZ_CP097594:1329710-1329991 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References